Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study

Spínola, Hélder; Bruges-Armas, Jácome; Brehm, António

http://hdl.handle.net/10400.13/1351

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Authors

Spínola, Hélder

Bruges-Armas, Jácome

Brehm, António

Abstract(s)

Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-speciﬁcoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identiﬁed by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difﬁculties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment.

Keywords

HLA Polymerase chain reaction - sequence-speciﬁc - oligonucleotide probe (PCR-SSOP) technique Sequence-based typing (SBT) technique Portugal Cabo Verde Guiné-Bissau Azores islands (Portugal) .

URI

http://hdl.handle.net/10400.13/1351

Citation

Spínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543.