Repository logo
 
Publication

Exploring the potential of needle trap microextraction combined with chromatographic and statistical data to discriminate different types of cancer based on urinary volatomic biosignature

2018Journal article Restricted access
Simple item page
dc.contributor.authorPorto-Figueira, Priscilla
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-26T13:17:42Z
dc.date.available2019-07-26T13:17:42Z
dc.date.issued2018
dc.description.abstractThe worldwide high cancer incidence and mortality demands for more effective and specific diagnostic strategies. In this study, we evaluated the efficiency of an innovative methodology, Needle Trap Microextraction (NTME), combined with gas chromatography-mass spectrometry (GC-MS), for the establishment of the urinary volatomic biosignature from breast (BC), and colon (CC) cancer patients as well as healthy individuals (CTL). To achieve this, 40 mL of the headspace of acidified urine (4 mL, 20% NaCl, pH = 2), equilibrated at 50 °C during 40 min, were loaded through the DVB/Car1000/CarX sorbent inside the NTD, and subjected to a GC-MS analysis. This allowed the identification of 130 VOMs from different chemical families that were further processed using discriminant analysis through the partial least squares method (PLS-DA). Several pathways are over activated in cancer patients, being phenylalanine pathway in BC and limonene and pinene degradation pathway in CC the most relevant. Butanoate metabolism is also highly activated in both cancers, as well as tyrosine metabolism in a lesser extension. In BC the xenobiotics metabolism by cytochrome P450 and fatty acid biosynthesis are also differentially activated. Different clusters corresponding to the groups recruited allowed to define sets of volatile organic metabolites (VOMs fingerprints) that exhibit high classification rates, sensitivity and specificity in the discrimination of the selected cancers. As far as we are aware, this is the first time that NTME is used for isolation urinary volatile metabolites, being the obtained results very promising.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPorto-Figueira, P., Pereira, J. A., & Camara, J. S. (2018). Exploring the potential of needle trap microextraction combined with chromatographic and statistical data to discriminate different types of cancer based on urinary volatomic biosignature. Analytica chimica acta, 1023, 53-63.pt_PT
dc.identifier.doi10.1016/j.aca.2018.04.027pt_PT
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/10400.13/2516
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.relationEXPLORING THE VOLATOME OF NONCOMMUNICABLE DISEASES AS A PROMISING, INNOVATIVE AND INTEGRATING APPROACH FOR THEIR RAPID DIAGNOSTICS. THE CASE STUDY OF CANCER AND NEURODEGENERATIVE DISEASES
dc.relationHNC-i3OMICS - Development of an innovative and integrated platform based on OMICS patterns towards the next generation of cancer diagnosis and management
dc.subjectUrinept_PT
dc.subjectVolatile organic metabolitespt_PT
dc.subjectBiomarkerspt_PT
dc.subjectCancerpt_PT
dc.subjectNeedle trap microextractionpt_PT
dc.subjectGas chromatography-mass spectrometrypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleExploring the potential of needle trap microextraction combined with chromatographic and statistical data to discriminate different types of cancer based on urinary volatomic biosignaturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardTitleEXPLORING THE VOLATOME OF NONCOMMUNICABLE DISEASES AS A PROMISING, INNOVATIVE AND INTEGRATING APPROACH FOR THEIR RAPID DIAGNOSTICS. THE CASE STUDY OF CANCER AND NEURODEGENERATIVE DISEASES
oaire.awardTitleHNC-i3OMICS - Development of an innovative and integrated platform based on OMICS patterns towards the next generation of cancer diagnosis and management
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/New-INDIGO%2F0003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/Inn-INDIGO%2F0001%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F129630%2F2017/PT
oaire.citation.endPage63pt_PT
oaire.citation.startPage53pt_PT
oaire.citation.titleAnalytica Chimica Actapt_PT
oaire.citation.volume1023pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
person.familyNamePorto-Figueira
person.familyNameAugusto Machado Pereira
person.familyNameCâmara
person.givenNamePriscilla
person.givenNameJorge
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idAD11-4860-FB1B
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0001-7773-182X
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id56724173300
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa617cf60-23e3-40ac-9ca8-f0d4b867d8e2
relation.isAuthorOfPublication2f4d7adf-ab6b-40ab-85d5-73995a784bda
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscoverya617cf60-23e3-40ac-9ca8-f0d4b867d8e2
relation.isProjectOfPublication75aaadc4-cd17-4fec-ba9d-1d0e564b3731
relation.isProjectOfPublication94bf6a05-292e-49da-a397-7a2bf175a1a9
relation.isProjectOfPublication31fd4a52-8b42-4c58-95a2-b4644fe10d9f
relation.isProjectOfPublicationd34b6935-25c8-479b-8520-9c310cf6d3e3
relation.isProjectOfPublication.latestForDiscovery94bf6a05-292e-49da-a397-7a2bf175a1a9

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Exploring the potential of needle trap microextraction combined with chromatographic and statistical data.pdf
Size:
2.21 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Artigos em revistas internacionais