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Publication
Phenolic screening by HPLC–DAD–ESI/MSn and antioxidant capacity of leaves, flowers and berries of Rubus grandifolius Lowe
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Advisor(s)
Abstract(s)
In Madeira Island (Macaronesia Island), Rubus grandifolius Lowe berries, locally known by amoras, are
widely consumed fresh or processed as jam, juice or liquor. Folk medicine describes R. grandifolius Lowe
fruits and leaves being used to treat diabetes, as depurative, diuretic and to relieve sore throat.
The aim of this study was to investigate phenolic composition and antioxidant capacity of the different
edible parts of the plant (berries, leaves and flowers). HPLC–DAD–ESI/MSn was used to establish the phe nolic profile. Phenolic monomers such as flavonol O-glycosilated (quercetin and kaempferol), quinic acid
and caffeic acid conjugates were characterized using the electrospray source in the negative mode; while
positive mode was employed to detect glycosylated anthocyanins (cyanidin, delphinin and petunidin).
The berries presented a higher radical scavenger capacity (DPPH and ABTS assays) and reducing prop erties (FRAP) than the leaves and the flowers. Ethanolic extracts showed highest antioxidant capacity
when compared with water based extracts: DPPH values of 147.9 ± 0.7 mol eq Trolox/g DM; ABTS value
of 255.8 ± 1.9 mol eq Trolox/g DM and FRAP value 9455 ± 29 mmol Fe(II)/mgDM).
Description
Keywords
Rubus Berries Phenolic compounds HPLC–DAD–ESI/MSn Antioxidant . Faculdade de Ciências Exatas e da Engenharia
Citation
Gouveia-Figueira, S. C., & Castilho, P. C. (2015). Phenolic screening by HPLC–DAD–ESI/MSn and antioxidant capacity of leaves, flowers and berries of Rubus grandifolius Lowe. Industrial Crops and Products, 73, 28-40.
Publisher
Elsevier