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Phenolic screening by HPLC–DAD–ESI/MSn and antioxidant capacity of leaves, flowers and berries of Rubus grandifolius Lowe

2015Journal article Open access
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dc.contributor.authorGouveia-Figueira, Sandra C.
dc.contributor.authorCastilho, Paula C.
dc.date.accessioned2021-03-29T13:32:57Z
dc.date.available2021-03-29T13:32:57Z
dc.date.issued2015
dc.description.abstractIn Madeira Island (Macaronesia Island), Rubus grandifolius Lowe berries, locally known by amoras, are widely consumed fresh or processed as jam, juice or liquor. Folk medicine describes R. grandifolius Lowe fruits and leaves being used to treat diabetes, as depurative, diuretic and to relieve sore throat. The aim of this study was to investigate phenolic composition and antioxidant capacity of the different edible parts of the plant (berries, leaves and flowers). HPLC–DAD–ESI/MSn was used to establish the phe nolic profile. Phenolic monomers such as flavonol O-glycosilated (quercetin and kaempferol), quinic acid and caffeic acid conjugates were characterized using the electrospray source in the negative mode; while positive mode was employed to detect glycosylated anthocyanins (cyanidin, delphinin and petunidin). The berries presented a higher radical scavenger capacity (DPPH and ABTS assays) and reducing prop erties (FRAP) than the leaves and the flowers. Ethanolic extracts showed highest antioxidant capacity when compared with water based extracts: DPPH values of 147.9 ± 0.7 mol eq Trolox/g DM; ABTS value of 255.8 ± 1.9 mol eq Trolox/g DM and FRAP value 9455 ± 29 mmol Fe(II)/mgDM).pt_PT
dc.description.sponsorshipMrs. Conceic¸ ão Pires is recognized for excellent assistance in sample collection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGouveia-Figueira, S. C., & Castilho, P. C. (2015). Phenolic screening by HPLC–DAD–ESI/MSn and antioxidant capacity of leaves, flowers and berries of Rubus grandifolius Lowe. Industrial Crops and Products, 73, 28-40.pt_PT
dc.identifier.doi10.1016/j.indcrop.2015.03.022pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3216
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationISOLAMENTO E CARACTERIZAÇÃO DE PRINCÍPIOS ACTIVOS DE PLANTAS PARA O COMBATE À TUBERCULOSE
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectRubuspt_PT
dc.subjectBerriespt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectHPLC–DAD–ESI/MSnpt_PT
dc.subjectAntioxidantpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titlePhenolic screening by HPLC–DAD–ESI/MSn and antioxidant capacity of leaves, flowers and berries of Rubus grandifolius Lowept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleISOLAMENTO E CARACTERIZAÇÃO DE PRINCÍPIOS ACTIVOS DE PLANTAS PARA O COMBATE À TUBERCULOSE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F24227%2F2005/PT
oaire.citation.endPage40pt_PT
oaire.citation.startPage28pt_PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume73pt_PT
person.familyNameGouveia-Figueira
person.familyNameCastilho
person.givenNameSandra
person.givenNamePaula C
person.identifierA-4412-2013
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0002-8116-2794
person.identifier.orcid0000-0002-8303-4286
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf17ed1c1-62a1-48be-8299-95e04f4a1c5c
relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
relation.isAuthorOfPublication.latestForDiscoveryf17ed1c1-62a1-48be-8299-95e04f4a1c5c
relation.isProjectOfPublicatione32bdc44-7898-4a45-be04-7048bfb66579
relation.isProjectOfPublication.latestForDiscoverye32bdc44-7898-4a45-be04-7048bfb66579

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