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Improved analytical approach for determination of tropane alkaloids in leafy vegetables based on ยต-QuEChERS combined with HPLC-MS/MS
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Orientador(es)
Resumo(s)
<jats:p>This work presents an optimized methodology based on the miniaturization of the original QuEChERS (ฮผ-QuEChERS) followed by liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) for the determination of tropane alkaloids (TAs), atropine, and scopolamine in leafy vegetable samples. The analytical methodology was successfully validated, demonstrating quantitation limits (MQL) โค 2.3 ng/g, good accuracy, and precision, with recoveries between 90โ100% and RSD โค 13% for both analytes. The method was applied to the analysis of TA-producing plants (Brugmansia versicolor, Solandra maxima, and Convolvulus arvensis). High concentrations of scopolamine were found in flowers (1771 mg/kg) and leaves (297 mg/kg) of B. versicolor. The highest concentration of atropine was found in flowers of S. maxima (10.4 mg/kg). Commercial mixed leafy vegetables contaminated with B. versicolor and S. maxima were analysed to verify the efficacy of the method, showing recoveries between 82 and 110% for both analytes. Finally, the method was applied to the analysis of eighteen samples of leafy vegetables, finding atropine in three samples of mixed leafy vegetables, with concentrations of 2.7, 3.2, and 3.4 ng/g, and in nine samples with concentrations โคMQL. In turn, scopolamine was only found in a sample of chopped Swiss chard with a concentration โคMQL.</jats:p>
Descriรงรฃo
Palavras-chave
Atropine Scopolamine Tropane alkaloids ยต-QuEChERS HPLC-MS/MS Leafy vegetables . Centro de Quรญmica da Madeira Faculdade de Ciรชncias Exatas e da Engenharia
Contexto Educativo
Citaรงรฃo
Gonzรกlez-Gรณmez, L.; Morante-Zarcero, S.; Pereira, J.A.M.; Cรขmara, J.S.; Sierra, I. Improved Analytical Approach for Determination of Tropane Alkaloids in Leafy Vegetables Based on ยต-QuEChERS Combined with HPLC-MS/MS. Toxins 2022, 14, 650. https://doi.org/ 10.3390/toxins14100650
Editora
MDPI