A fast and environment-friendly MEPS PEP /UHPLC-PDA methodology to assess 3-hydroxy-4,5-dimethyl-2(5H)-furanone in fortified wines

Freitas, Jorge; Perestrelo, Rosa; Cassaca, Rubina; Castillo, Mariangie; Santos, Mariana; Pereira, Jorge; Câmara, José S.

http://hdl.handle.net/10400.13/2505

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
A fast and environment friendly MEPSPEP UHPLC-PDA methodology.pdf		1.03 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Sotolon is widely associated with the quality of fortified aged wines, and has also been linked to premature oxidative aging (premox). Here we developed a single, fast and environmental-friendly microextraction by packed sorbent ultra-high pressure liquid chromatography analysis (MEPS/UHPLC-PDA) for sotolon quantification in different wines. The best extraction conditions (loading three times 250μL samples through the MEPSPEP sorbent and elution with 100μL of 50% MeOH) were combined with a fast UHPLC separation (5min separation using acidified 10% MeOH isocratic flow in a CORTECS C18 column) to allow unparalleled minimum sample and solvents volumes usage. The validated methodology showed good linearity (r(2)>0.993) and precision (<5.6%); high recovery (>81%) and detection limits (0.45-2.51μg/L) far below sotolon odor threshold for any type of wine. The methodology was successfully applied to selected white table and Madeira wines, encompassing therefore a wide range of alcohol and sugar contents. Furthermore, as far we may know, this is the first time a single methodology can be used to assess both wine aging or premox according to the type of wine.

Keywords

Sotolon Wine Oxidative aging Premox Microextraction by packed sorbent (MEPS) . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira

URI

http://hdl.handle.net/10400.13/2505

Citation

Freitas, J., Perestrelo, R., Cassaca, R., Castillo, M., Santos, M., Pereira, J., & Câmara, J. S. (2017). A fast and environment-friendly MEPSPEP/UHPLC-PDA methodology to assess 3-hydroxy-4, 5-dimethyl-2 (5H)-furanone in fortified wines. Food chemistry, 214, 686-693.