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Comparative lipidomic analysis of Chlorella stigmatophora and Hemiselmis cf. andersenii in response to nitrogen-induced changes

2021Journal article Open access
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dc.contributor.authorFernandes, Tomásia
dc.contributor.authorFerreira, Artur
dc.contributor.authorCordeiro, Nereida
dc.date.accessioned2022-02-09T15:48:40Z
dc.date.available2022-02-09T15:48:40Z
dc.date.issued2021
dc.description.abstractThe current focus of algae biotechnology is the production of high-value lipids, and its improvement by employing abiotic perturbations such as nitrogen-induced changes. In the present study, the growth dynamics, nitrogen uptake, pigments, and lipid composition of Chlorellla stigmatophora and Hemiselmis cf. andersenii were studied, in response to low (LN), medium (MN) and high (HN) nitrogen supplementations. Both microalgae responded to increased nitrogen levels by increasing their nitrogen uptake rate and pigment content. However, for lipid accumulation, C. stigmatophora presented a different pattern (LN: 16.56% > MN: 11.51% > HN: 10.95%) to that of H. cf. andersenii (MN: 15.37% > HN: 13.06% > LN: 6.71%). Untargeted gas chromatography–mass spectrometry analysis allowed the visualization of the biochemical diversity of C. stigmatophora and H. cf. andersenii, as well as differences in lipid regulation upon nitrogen-induced changes among species. For instance, glycosyl sterols were only detected for C. stigmatophora samples grown under MN and HN conditions. Moreover, lipid analysis of H. cf. andersenii, before and after alkaline hydrolysis, suggests that wax esters play a key role in the response of this microalga to high nitrogen levels. The cultivation of H. cf. andersenii at MN and HN was shown to be ideal for providing a rich source of ω3 and polyunsaturated fatty acids for nutraceutical purposes. The hierarchical cluster analysis showed the differential intra- and interspecific effects of nitrogen on lipid composition. The diverse ways by which both microalgae responded to nitrogen-induced changes highlighted the influence of phylogeny on the carbon flux through metabolic networks, and accumulation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, T., Ferreira, A., & Cordeiro, N. (2021). Comparative lipidomic analysis of Chlorella stigmatophora and Hemiselmis cf. andersenii in response to nitrogen-induced changes. Algal Research, 58, 102417.pt_PT
dc.identifier.doi10.1016/j.algal.2021.102417pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4069
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectNitrogen supplementationpt_PT
dc.subjectHemiselmis cf. anderseniipt_PT
dc.subjectChlorella stigmatophorapt_PT
dc.subjectLipidome analysispt_PT
dc.subjectNutraceutical lipidspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleComparative lipidomic analysis of Chlorella stigmatophora and Hemiselmis cf. andersenii in response to nitrogen-induced changespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.citation.startPage102417pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume58pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameGomez Fernandes
person.familyNamecordeiro
person.givenNameTomásia Micaela
person.givenNamenereida
person.identifier1612636
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0002-6157-4177
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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