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Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD–ESI-MSn and screening for their antioxidant activity

2015Journal article Open access
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dc.contributor.authorSpínola, Vítor
dc.contributor.authorPinto, Joana
dc.contributor.authorCastilho, Paula C.
dc.date.accessioned2021-03-30T09:18:23Z
dc.date.available2021-03-30T09:18:23Z
dc.date.issued2015
dc.description.abstractFive fruits species commonly cultivated and consumed in Madeira Island (Portugal) were investigated for their phenolic profile by means of reversed phase high-performance liquid chromatography coupled to diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS(n)) and antioxidant potential. A large number of compounds were characterised, flavonoids and phenolic acids being the major components found in target samples, 39 compounds (flavonoids, phenolic acids, terpenoids, cyanogenic glycosides and organic acids) were identified in cherimoyas, lemons, papayas, passion-fruits and strawberries for the first time. Furthermore, all samples were systematically analysed for their total phenolic and flavonoid contents along with two radical scavenging methods (ABTS and ORAC) for antioxidant activity measurement. Target fruits presented high phenolic contents which is responsible for most of the antioxidant activity against radical reactive species (R(2)>0.80). Quantitative data showed that anthocyanins, in particular pelargonidin-3-O-hexoside (>300 mg/100 mL), present only in strawberries were the compounds in largest amounts but are the ones which contribute less to the antioxidant activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSpínola, V., Pinto, J., & Castilho, P. C. (2015). Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD–ESI-MSn and screening for their antioxidant activity. Food Chemistry, 173, 14-30.pt_PT
dc.identifier.doi10.1016/j.foodchem.2014.09.163pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3219
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectHPLC-DAD–ESI/MSnpt_PT
dc.subjectFruits juicept_PT
dc.subjectQuantificationpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleIdentification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD–ESI-MSn and screening for their antioxidant activitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage30pt_PT
oaire.citation.startPage14pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume173pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSpínola
person.familyNameNunes Pinto
person.familyNameCastilho
person.givenNameVítor
person.givenNameJoana Carolina
person.givenNamePaula C
person.identifierA-4412-2013
person.identifier.ciencia-id0412-6D53-3A09
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0003-2456-8613
person.identifier.orcid0000-0002-8303-4286
person.identifier.scopus-author-id54881258000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6bf71312-4006-4df6-9fbe-d9ed8ce12b06
relation.isAuthorOfPublication9f82af5c-7427-4e62-80ac-4c4feb2f615a
relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
relation.isAuthorOfPublication.latestForDiscovery9f82af5c-7427-4e62-80ac-4c4feb2f615a
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