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Molecular cloning and characterization of cDNAs encoding cytosolic malate dehydrogenase and vacuolar (H+)-ATPase in Annona cherimola and their expression during postharvest ripening

2018Journal article Open access
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dc.contributor.authorGouveia, Manuela
dc.contributor.authorRodrigues, Mónica
dc.contributor.authorTeixeira, Lucília
dc.contributor.authorCordeiro, Nereida
dc.date.accessioned2021-04-22T13:25:28Z
dc.date.available2021-04-22T13:25:28Z
dc.date.issued2018
dc.description.abstractThis study aims to investigate the expression of two cherimoya genes putatively related to fruit ripening. Two full-length cDNAs encoding cytosolic NAD-dependent malate dehydrogenase (AccytMDH) and vacuolar (H+)-ATPase c subunit (AcVHA-c) were isolated from Annona cherimola using the reverse transcriptase polymerase chain reaction (RT- PCR) and rapid amplification of cDNA ends (RACE). The AccytMDH codes for a 332 amino acid polypeptide with a predicted molecular mass of 35.6 kDa. The deduced amino acid sequence for AccytMDH shared high identity with other plant homologous malate dehydrogenase proteins. The AcVHA-c encodes a proteolipid subunit of the V-type proton ATPase with 166 amino acids (16.7 kDa). Comparison of the deduced amino acid sequence from AcVHA-c revealed four transmembrane domains highly conserved among plant counterparts. The expression of AccytMDH and AcVHA-c, assessed by semi-quantitative RT-PCR showed that there is an increase in the accumulation of transcripts during postharvest ripening, although not correlated by a significant upsurge of titratable acidity they might contribute to organic acid accumulation and translocation during postharvest ripening of cherimoya in association with other enzymes and carriers. By using AccytMDH and AcVHA-c as molecular targets new strategies can be exploited to get a clear picture in the ripening of cherimoya.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGouveia, M., Rodrigues, M., Teixeira, L., & Cordeiro, N. (2018). Molecular cloning and characterization of cDNAs encoding cytosolic malate dehydrogenase and vacuolar (H+)-ATPase in Annona cherimola and their expression during postharvest ripening. Indian Journal of Biotechnology, 17, 422-430.pt_PT
dc.identifier.issn0975-0967
dc.identifier.urihttp://hdl.handle.net/10400.13/3312
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNISCAIR-CSIR, Indiapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCherimoyapt_PT
dc.subjectAnnona cherimolapt_PT
dc.subjectFruit ripeningpt_PT
dc.subjectGene expressionpt_PT
dc.subjectMalate dehydrogenasept_PT
dc.subjectVacuolar ATPasept_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectFaculdade de Ciências da Vida
dc.titleMolecular cloning and characterization of cDNAs encoding cytosolic malate dehydrogenase and vacuolar (H+)-ATPase in Annona cherimola and their expression during postharvest ripeningpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage430pt_PT
oaire.citation.startPage422pt_PT
oaire.citation.titleIndian Journal of Biotechnologypt_PT
oaire.citation.volume17pt_PT
person.familyNameGouveia
person.familyNameRodrigues
person.familyNamecordeiro
person.givenNameManuela
person.givenNameMónica
person.givenNamenereida
person.identifier1612636
person.identifier.ciencia-idA017-CFB6-50D5
person.identifier.ciencia-idA51D-6EE7-115D
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0001-5758-2230
person.identifier.orcid0000-0001-7011-8723
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id16480248200
person.identifier.scopus-author-id7004319456
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione7211b14-d59d-49b9-8a5c-b44291e0ea77
relation.isAuthorOfPublicatione94e273e-bae2-44b0-bbfd-732c314d6c65
relation.isAuthorOfPublication3d02b657-c48c-4f56-813a-1284b81960f3
relation.isAuthorOfPublication.latestForDiscoverye94e273e-bae2-44b0-bbfd-732c314d6c65

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