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An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

2012Journal article Open access
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dc.contributor.authorSpínola, Vítor
dc.contributor.authorMendes, Berta
dc.contributor.authorCâmara, José S.
dc.contributor.authorCastilho, Paula C.
dc.date.accessioned2021-03-31T10:11:10Z
dc.date.available2021-03-31T10:11:10Z
dc.date.issued2012
dc.description.abstractThis study provides a versatile validated method to determine the total vitamin C content, as the sum of the contents of L-ascorbic acid (L-AA) and dehydroascorbic acid (DHAA), in several fruits and vegetables and its degradability with storage time. Seven horticultural crops from two different origins were analyzed using an ultra-high-performance liquid chromatographic-photodiode array (UHPLC-PDA) system, equipped with a new trifunctional high strength silica (100% silica particle) analytical column (100 mm × 2.1 mm, 1.7 μm particle size) using 0.1% (v/v) formic acid as mobile phase, in isocratic mode. This new stationary phase, specially designed for polar compounds, overcomes the problems normally encountered in HPLC and is suitable for the analysis of large batches of samples without L-AA degradation. In addition, it proves to be an excellent alternative to conventional C18 columns for the determination of L-AA in fruits and vegetables. The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, accuracy, and inter/intra-day precision. Validation experiments revealed very good recovery rate of 96.6 ± 4.4% for L-AA and 103.1 ± 4.8 % for total vitamin C, good linearity with r(2)-values >0.999 within the established concentration range, excellent repeatability (0.5%), and reproducibility (1.6%) values. The LOD of the method was 22 ng/mL whereas the LOQ was 67 ng/mL. It was possible to demonstrate that L-AA and DHAA concentrations in the different horticulture products varied oppositely with time of storage not always affecting the total amount of vitamin C during shelf-life. Locally produced fruits have higher concentrations of vitamin C, compared with imported ones, but vegetables showed the opposite trend. Moreover, this UHPLC-PDA methodology proves to be an improved, simple, and fast approach for determining the total content of vitamin C in various food commodities, with high sensitivity, selectivity, and resolving power within 3 min of run analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSpínola, V., Mendes, B., Câmara, J. S., & Castilho, P. C. (2012). An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage. Analytical and bioanalytical chemistry, 403(4), 1049-1058.pt_PT
dc.identifier.doi10.1007/s00216-011-5668-xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3229
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAscorbic acidpt_PT
dc.subjectDehydroascorbic acidpt_PT
dc.subjectTotal vitamin Cpt_PT
dc.subjectUltra-high-performance liquid chromatographypt_PT
dc.subjectValidationpt_PT
dc.subjectFruits and vegetablespt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAn improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage1058pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1049pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume403pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSpínola
person.familyNameCâmara
person.familyNameCastilho
person.givenNameVítor
person.givenNameJosé
person.givenNamePaula C
person.identifierG-3003-2013
person.identifierA-4412-2013
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0003-2456-8613
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-8303-4286
person.identifier.scopus-author-id54881258000
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
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