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Improved analytical approach based on QuECHERS/UHPLC-PDA for quantification of fluoxetine, clomipramine and their active metabolites in human urine samples

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dc.contributor.authorAlves, Vera
dc.contributor.authorConceição, Carlota
dc.contributor.authorGonçalves, João
dc.contributor.authorTeixeira, Helena M.
dc.contributor.authorCâmara, José
dc.date.accessioned2019-07-25T08:56:01Z
dc.date.available2019-07-25T08:56:01Z
dc.date.issued2017
dc.description.abstractThis paper reports, for the first time, the development of a modified Quick, Easy, Cheap Effective, Rugged and Safe (QuEChERS) combined with a dispersive SPE (d-SPE) based clean-up procedure as a new and powerful strategy for the simultaneous and efficient isolation of two different antidepressants, fluoxetine and clomipramine, and their active metabolites in human urine samples. A univariate experimental design with four independent variables such as sample volume, extraction solvent, buffered salts and clean-up step, was performed and used to investigate the effect of process variables on the extraction efficiency. Good linearity was achieved at the studied concentration range (0.1-5.0 µg mL-1), with correlation coefficients (R2) higher than 0.9961. Low detection limits, ranging between 0.060 and 0.092 μg mL-1 were obtained for all analytes, whereas the lowest quantification limit was 0.1 μg mL-1, corresponding to the lowest concentration of the standard curve. The method also showed good results for accuracy, with values ranging from 91% to 105%. Intra- and inter-day precision, expressed as the relative standard deviation (RSD), were also satisfactory (<10%). Consistent recoveries of antidepressants ranging from 86% to 109% were observed when urine samples were fortified at three concentrations, namely 0.1, 2.5 and 5.0 μg mL-1 In order to evaluate the proposed method for clinical use, the QuEChERS/UHPLC-PDA method was applied to analysis of 12 urine samples from depressed patients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlves, V., Conceição, C., Gonçalves, J., Teixeira, H. M., & Câmara, J. S. (2017). Improved analytical approach based on QuECHERS/UHPLC-PDA for quantification of fluoxetine, clomipramine and their active metabolites in human urine samples. Journal of analytical toxicology, 41(1), 45-53.pt_PT
dc.identifier.doi10.1093/jat/bkw077pt_PT
dc.identifier.issn0146-4760
dc.identifier.urihttp://hdl.handle.net/10400.13/2508
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.subjectAntidepressive agentspt_PT
dc.subjectCalibrationpt_PT
dc.subjectClomipraminept_PT
dc.subjectFluoxetinept_PT
dc.subjectLimit of detectionpt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectReproducibility of resultspt_PT
dc.subjectQuECHERS/UHPLC-PDApt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleImproved analytical approach based on QuECHERS/UHPLC-PDA for quantification of fluoxetine, clomipramine and their active metabolites in human urine samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage53pt_PT
oaire.citation.startPage45pt_PT
oaire.citation.titleJournal of Analytical Toxicologypt_PT
oaire.citation.volume41(1)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAlves
person.familyNameGonçalves
person.familyNameCâmara
person.givenNameVera
person.givenNameJoão
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idE515-0AFA-CB1D
person.identifier.ciencia-id8C14-3188-9E44
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-1485-6032
person.identifier.orcid0000-0002-1265-6686
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridC-1348-2019
person.identifier.scopus-author-id55792994000
person.identifier.scopus-author-id52163735200
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication7971c475-3f08-4462-96b1-79e96c9615a4
relation.isAuthorOfPublicatione10d78be-e547-4d25-92b5-06a997ed78da
relation.isAuthorOfPublication.latestForDiscovery7971c475-3f08-4462-96b1-79e96c9615a4
relation.isProjectOfPublication75aaadc4-cd17-4fec-ba9d-1d0e564b3731
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