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An exploratory study to evaluate the potential of nanohydroxyapatite as a powerful sorbent for efficient extraction of volatile organic metabolites, potential biomarkers of cancer

2014Journal article Restricted access
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dc.contributor.authorQiao, Zheng
dc.contributor.authorPerestrelo, Rosa
dc.contributor.authorShi, Xiangyang
dc.contributor.authorRodrigues, João
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-06-21T13:17:45Z
dc.date.available2019-06-21T13:17:45Z
dc.date.issued2014
dc.description.abstractDeveloping early-stage diagnostic methods which are specific, highly sensitive and non-invasive for cancer has received much attention. In this paper, nanohydroxyapatite (NHA) was for the first time used as a sorbent to extract volatile organic metabolites (VOMs) which are considered as potential biomarkers of cancer, such as hexanal, heptanal, decanal, benzaldehyde, 4-heptanone, phenol, undecane, and 5-methyl-2-furfural. The extraction process was performed by simply dispersing nanohydroxyapatite into working solution containing targeted VOMs and then eluting the centrifuged sorbent with an organic solvent. Batch techniques were performed to optimize the experimental variables affecting the extraction of targeted VOMs such as the sorbent amount, adsorption time, elution time and types of elution solvent. The eluent was collected, concentrated and analyzed by gas chromatography-mass spectrometry (GC-qMS). Under optimized conditions, the results obtained demonstrated a good linearity (r2 ≥ 0.993), over the linear dynamic range, for all urinary volatile metabolites investigated. The proposed strategy provided limits of detection (LODs) ranging from 9.8 ng L−1 to 69.5 ng L−1 and limits of quantification (LOQs) from 32.5 ng L−1 to 231.6 ng L−1. The method also afforded satisfactory results in terms of the matrix effect (72.8–96.1%) and recoveries (accuracy) higher than 70% for most of the studied VOMs. The intra-day and inter-day precision was lower than 3% and 13%, respectively. The established method has been successfully applied to the determination of the target urinary VOMs, from cancer patients, described as potential cancer biomarkers.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationQiao, Z., Perestrelo, R., Shi, X., Rodrigues, J., & Câmara, J. S. (2014). An exploratory study to evaluate the potential of nanohydroxyapatite as a powerful sorbent for efficient extraction of volatile organic metabolites, potential biomarkers of cancer. Analytical Methods, 6(15), 6051-6057.pt_PT
dc.identifier.doi10.1039/C4AY00997ept_PT
dc.identifier.issn1759-9660
dc.identifier.urihttp://hdl.handle.net/10400.13/2417
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationStrategic Project - UI 674 - 2011-2012
dc.subjectNanohydroxyapatitept_PT
dc.subjectVolatile organic metabolitespt_PT
dc.subjectBiomarkers of cancerpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleAn exploratory study to evaluate the potential of nanohydroxyapatite as a powerful sorbent for efficient extraction of volatile organic metabolites, potential biomarkers of cancerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2011/PT
oaire.citation.endPage6057pt_PT
oaire.citation.startPage6051pt_PT
oaire.citation.titleAnalytical Methodspt_PT
oaire.citation.volume6(15)pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePerestrelo
person.familyNameShi
person.familyNameRodrigues
person.familyNameCâmara
person.givenNameRosa
person.givenNameXiangyang
person.givenNameJoão
person.givenNameJosé
person.identifier1441319
person.identifierG-3003-2013
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.ciencia-idBA11-1437-B948
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0002-7223-1022
person.identifier.orcid0000-0001-6785-6645
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0003-1965-3151
person.identifier.ridI-2307-2014
person.identifier.ridA-1289-2007
person.identifier.ridB-6816-2008
person.identifier.scopus-author-id16686828800
person.identifier.scopus-author-id7402953116
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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