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Advisor(s)
Abstract(s)
Understanding the biochemical and antioxidant composition and capacity of a target biomass is the first step to its
selectivity as functional food which can enhance the ability to promote health by reducing the risk of chronic diseases.
The main purpose of this work was to employ response surface methodology (RSM) to determine the effect of the
independent variables, % of ethanol (50–96%), time of sonication (0–20 min), and number of extraction cycles (6–18
cycles) in the primary extract of brown seaweed Zonaria tournefortii, studying the yield variation of some bioactive
compounds, assessing the potential of these bioextracts to integrate as a natural additives or supplements in the functional
food industry. The extractions were performed employing “Green Chemistry” techniques executed with the Timatic
extractor, which applied pressurized ethanol solution at a maximum pressure of 8.5 bar through milled dehydrated biomass
in the extraction vessel. Several parameters were assessed in the primary bioactive extract which included extract yield
(11.56–28.49 g (100 g)−1 dw), total chlorophyll content (0.14–1.42 g (100 g)−1 dw), total carotenoid content (0.35–0.80 g
(100 g)−1 dw), total fucoxanthin content (0.04–0.13 g (100 g)−1 dw), total phenolic content (3.58–5.84 g (100 g)−1 dw),
total flavonoid content (0.22–4.70 g (100 g)−1 dw), DPPH (56.05–76.45%), and reducing activity (3.83–6.04 g (100 g)−1
dw). A second objective was to determine the suitability of the residue for subsequent extraction of valuable compounds
such as fucoidan (4.87 to 6.59 g (100 g)−1 dw) and cellulose (18.88 to 20.27 g (100 g)−1 dw), implementing the first step to
a biorefinery strategy, using a cascade approach.
Description
Keywords
Functional food Bioactive extract Biorefinery Phaeophyceae Fucoidan . Faculdade de Ciências da Vida
Citation
Nunes, N., Valente, S., Ferraz, S., Barreto, M. C., & Carvalho, M. P. (2020). Constructing ethanol-derived bioactive extracts using the brown seaweed Zonaria tournefortii (JV Lamouroux) Montagne performed with Timatic extractor by means of response surface methodology (RSM). Journal of Applied Phycology, 32(4), 2321-2333. https://doi.org/10.1007/s10811-019-01973-9
Publisher
Springer