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A sensitive method for the rapid determination of underivatized ethyl carbamate in fortified wine by liquid chromatography-electrospray tandem mass spectrometry

2017Journal article Open access
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dc.contributor.authorLeça, João M.
dc.contributor.authorPereira, Vanda
dc.contributor.authorPereira, Ana C.
dc.contributor.authorMarques, José C.
dc.date.accessioned2021-10-14T07:53:37Z
dc.date.available2021-10-14T07:53:37Z
dc.date.issued2017
dc.description.abstractThis work presents the optimization of a miniaturized liquid-liquid extraction (mLLE) followed by reversed-phase liquid chromatography-electrospray tandem mass spectrometry (RP-HPLC-MS/MS) for the determination of ethyl carbamate (EC) in fortified wine, without using derivatizing agents. The mLLE was optimized by an experimental design. Thus, 15 mL of wine and 8 mL of ethyl acetate were used for the extraction. After concentration, each extract was injected into the HPLC MS/MS equipment and the characteristic secondary ion transition of EC (m/z = 90.10 → 62.05) was used for the quan tification purposes. The proposed method presented a good lin earity (R2 = 0.9999) and a high sensitivity with low limits of detection (LOD) and quantification (LOQ), 0.17 and 0.52 μg L−1 , respectively. The precision (repeatability and reproducibility) never exceeded 8% of variation, and the recoveries varied between 93 and 114%. The applicability of the method was checked through the analysis of 24 fortified wines, with EC values ranging between 23 ± 1 and 194 ± 5 μg L−1 . All chromatograms revealed good peak resolutions. This new method is efficient for the simple, fast, and reliable determination of EC in fortified wines, providing great sensitivity without using derivatizing agents or large volumes of organic solvents.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLeça, J. M., Pereira, V., Pereira, A. C., & Marques, J. C. (2018). A sensitive method for the rapid determination of underivatized ethyl carbamate in fortified wine by liquid chromatography-electrospray tandem mass spectrometry. Food analytical methods, 11(2), 327-333. DOI 10.1007/s12161-017-1002-3pt_PT
dc.identifier.doi10.1007/s12161-017-1002-3pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3725
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEthyl carbamatept_PT
dc.subjectLiquid-liquid extractionpt_PT
dc.subjectWinept_PT
dc.subjectLiquid chromatography-mass spectrometrypt_PT
dc.subjectDesign of experimentspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleA sensitive method for the rapid determination of underivatized ethyl carbamate in fortified wine by liquid chromatography-electrospray tandem mass spectrometrypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage333pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage327pt_PT
oaire.citation.titleFood Analytical Methodspt_PT
oaire.citation.volume11pt_PT
person.familyNameLeça
person.familyNamePereira
person.familyNamePereira
person.familyNameMarques
person.givenNameJoão Micael
person.givenNameVanda
person.givenNameAna Cristina
person.givenNameJose C
person.identifier1083569
person.identifier41146
person.identifier.ciencia-id6213-EB73-1A9F
person.identifier.ciencia-id0115-071E-87E8
person.identifier.ciencia-id251D-B696-A3D1
person.identifier.ciencia-id1713-6DA1-A551
person.identifier.orcid0000-0003-1704-6745
person.identifier.orcid0000-0003-0772-8953
person.identifier.orcid0000-0003-3097-7704
person.identifier.orcid0000-0002-4832-4341
person.identifier.ridJ-8697-2013
person.identifier.ridA-7681-2015
person.identifier.ridF-3014-2010
person.identifier.scopus-author-id55979990800
person.identifier.scopus-author-id36857887700
person.identifier.scopus-author-id56483357000
person.identifier.scopus-author-id7203033313
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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