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Publication
A systematic AQbD approach for optimization of the most influential experimental parameters on analysis of fish spoilage-related volatile amines
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Advisor(s)
Abstract(s)
The volatile amines trimethylamine (TMA) and dimethylamine (DMA) could be used
as important spoilage indices for seafood products, assisting in the determination of the rejection
period. In the present study, a systematic analytical duality-by-design (AQbD) approach was used as
a powerful strategy to optimize the most important experimental parameters of headspace solid-phase
microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) conditions for
the quantification of TMA and DMA in Sparus aurata. This optimization enabled the selection of
the best points in the method operable design region for HS-SPME extraction (30 min; 35 ◦C; NaOH
15 M and NaCl 35%, w/v) and GC-MS analysis (80 ◦C; gradient 50 ◦C/min; flow rate 1 mL/min and
splitless mode). The rejection day, estimated through the TMA concentration (>12 mg/100 g, at
days 9–10), was compared with sensory (quality index method: day 7–8), physical (Torrymeter: day
8–9), and microbial (day 9–10) analysis, corroborating the suitability of the proposed approach for
estimating the period for which they will retain an acceptable level of eating quality from a safety
and sensory perspective.
Description
Keywords
Sparus aurata Trimethylamine Dimethylamine Freshness HS-SPME GC-MS Microbiology Dielectric properties Sensory analysis . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Freitas, J., Silva, P., Vaz-Pires, P., & Câmara, J. S. (2020). A systematic AQbD approach for optimization of the most influential experimental parameters on analysis of fish spoilage-related volatile amines. Foods, 9(9), 1321.
Publisher
MDPI