Publication
Headspace solid-phase microextraction combined with mass spectrometry as a powerful analytical tool for proļ¬ling the terpenoid metabolomic pattern of hop-essential oil derived from Saaz variety
| dc.contributor.author | GonƧalves, JoĆ£o | |
| dc.contributor.author | Figueira, JosƩ S. | |
| dc.contributor.author | Rodrigues, FƔtima | |
| dc.contributor.author | CĆ¢mara, JosĆ© | |
| dc.date.accessioned | 2015-12-10T14:55:11Z | |
| dc.date.available | 2015-12-10T14:55:11Z | |
| dc.date.issued | 2012-05 | |
| dc.description.abstract | Hop(HumuluslupulusL.,Cannabaceaefamily)isprizedforitsessentialoilcontents,usedin beer production and, more recently, in biological and pharmacological applications. In this work,a methodinvolvingheadspace solid-phase microextractionand gas chromatographyā mass spectrometry was developed and optimized to establish the terpenoid (monoterpenes and sesquiterpenes) metabolomic pattern of hop-essential oil derived from Saaz variety as a mean to explore this matrix as a powerful biological source for newer, more selective, biodegradable and naturally produced antimicrobial and antioxidant compounds. Different parameters affecting terpenoid metabolites extraction by headspace solid-phase microextraction were considered and optimized: type of ļ¬ber coatings, extraction temperature, extraction time, ionic strength, and sample agitation. In the optimized method, analytes were extracted for 30 min at 40 C in the sample headspace with a 50/30 m divinylbenzene/carboxen/polydimethylsiloxane coating ļ¬ber. The methodology allowed the identiļ¬cation of a total of 27 terpenoid metabolites, representing 92.5% of the total Saaz hop-essential oil volatile terpenoid composition. The headspace composition was dominated by monoterpenes (56.1%, 13 compounds), sesquiterpenes (34.9%, 10), oxygenated monoterpenes (1.41%, 3), and hemiterpenes (0.04%, 1) some of which can probably contribute to the hop of Saaz variety aroma. Mass spectrometry analysis revealed that the main metabolites are the monoterpene -myrcene (53.0Ā±1.1% of the total volatile fraction), and the cyclic sesquiterpenes, -humulene (16.6 Ā± 0.8%), and -caryophyllene (14.7 Ā± 0.4%), which together represent about 80% of the total volatile fraction from the hop-essential oil. Theseļ¬ndingssuggestthatthismatrixcanbeexploredasapowerfulbiosourceofterpenoid metabolites. | pt_PT |
| dc.identifier.citation | GonƧalves, J., Figueira, J., Rodrigues, F., & Camara, J. S. (2012). Headspace solidāphase microextraction combined with mass spectrometry as a powerful analytical tool for profiling the terpenoid metabolomic pattern of hopāessential oil derived from Saaz variety. Journal of separation science, 35(17), 2282-2296. | pt_PT |
| dc.identifier.doi | 10.1002/jssc.201200244 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.13/948 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | WILEY-VCH Verlag GmbH & Co. KGaA | pt_PT |
| dc.subject | Essential oil | pt_PT |
| dc.subject | GC-qMS | pt_PT |
| dc.subject | HopSaaz variety | pt_PT |
| dc.subject | HS-SPME | pt_PT |
| dc.subject | Terpenoid metabolites | pt_PT |
| dc.subject | . | pt_PT |
| dc.subject | Faculdade de CiĆŖncias Exatas e da Engenharia | pt_PT |
| dc.subject | Centro de QuĆmica da Madeira | |
| dc.title | Headspace solid-phase microextraction combined with mass spectrometry as a powerful analytical tool for proļ¬ling the terpenoid metabolomic pattern of hop-essential oil derived from Saaz variety | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 2296 | pt_PT |
| oaire.citation.startPage | 2282 | pt_PT |
| oaire.citation.title | Journal of separation science | pt_PT |
| oaire.citation.volume | 35(17) | pt_PT |
| person.familyName | GonƧalves | |
| person.familyName | Oliveira Figueira | |
| person.familyName | CĆ¢mara | |
| person.givenName | JoĆ£o | |
| person.givenName | JosĆ© AldĆ³nio | |
| person.givenName | JosƩ | |
| person.identifier | A-8724-2017 | |
| person.identifier | G-3003-2013 | |
| person.identifier.ciencia-id | 8C14-3188-9E44 | |
| person.identifier.ciencia-id | 0B1E-B3DD-95F5 | |
| person.identifier.ciencia-id | 481C-08CE-90E5 | |
| person.identifier.orcid | 0000-0002-1265-6686 | |
| person.identifier.orcid | 0000-0003-3719-9327 | |
| person.identifier.orcid | 0000-0003-1965-3151 | |
| person.identifier.rid | C-1348-2019 | |
| person.identifier.scopus-author-id | 52163735200 | |
| person.identifier.scopus-author-id | 57201813505 | |
| person.identifier.scopus-author-id | 10140393000 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 7971c475-3f08-4462-96b1-79e96c9615a4 | |
| relation.isAuthorOfPublication | fc7f1e5e-1f91-4dd7-993c-f0e2063cc8c8 | |
| relation.isAuthorOfPublication | e10d78be-e547-4d25-92b5-06a997ed78da | |
| relation.isAuthorOfPublication.latestForDiscovery | 7971c475-3f08-4462-96b1-79e96c9615a4 |
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