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Publication
Multiplexed-shotgun-genotyping data resolve phylogeny within a very recently derived insular lineage
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Advisor(s)
Abstract(s)
Premise of the study: Endemic plants on oceanic islands have long served as model systems for studying patterns and processes
of evolution. However, phylogenetic studies of island plants frequently illustrate a decoupling of molecular divergence and
ecological/morphological diversity, resulting in phylogenies lacking the resolution required to interpret patterns of evolution in
a phylogenetic context. The current study uses the primarily Macaronesian fl owering plant genus Tolpis to illustrate the utility
of multiplexed shotgun genotyping (MSG) for resolving relationships at relatively deep (among archipelagos) and very shallow
(within archipelagos) nodes in this small, yet diverse insular plant lineage that had not been resolved with other molecular
markers.
• Methods: Genomic libraries for 27 accessions of Macaronesian Tolpis were generated for genotyping individuals using MSG,
a form of reduced-representation sequencing, similar to restriction-site-associated DNA markers (RADseq). The resulting data
fi les were processed using the program pyRAD, which clusters MSG loci within and between samples. Phylogenetic analyses
of the aligned data matrix were conducted using RAxML.
• Key results: Analysis of MSG data recovered a highly resolved phylogeny with generally strong support, including the fi rst
robust inference of relationships within the highly diverse Canary Island clade of Tolpis .
• Conclusions: The current study illustrates the utility of MSG data for resolving relationships in lineages that have undergone
recent, rapid diversifi cation resulting in extensive ecological and morphological diversity. We suggest that a similar approach
may prove generally useful for other rapid plant radiations where resolving phylogeny has been diffi cult.
Description
Keywords
Asteraceae Canary Islands (Spain) Genome sequencing Macaronesia Multiplexed shotgun genotyping Phylogenomics Phylogeny Radiation Tolpis . Faculdade de Ciências da Vida
Citation
Mort, M. E., Crawford, D. J., Kelly, J. K., Santos‐Guerra, A., Sequeira, M. M., Moura, M., & Caujapé‐Castells, J. (2015). Multiplexed‐shotgun‐genotyping data resolve phylogeny within a very recently derived insular lineage. American Journal of Botany, 102(4), 634-641. https://doi.org/10.3732/ajb.1400551
Publisher
Botanical Society of America