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Multiplexed-shotgun-genotyping data resolve phylogeny within a very recently derived insular lineage

2015Journal article Open access
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dc.contributor.authorMort, Mark E.
dc.contributor.authorCrawford, Daniel J.
dc.contributor.authorKelly, John K.
dc.contributor.authorSantos-Guerra, Arnoldo
dc.contributor.authorSequeira, Miguel Menezes de
dc.contributor.authorMoura, Mónica
dc.contributor.authorCaujapé-Castells, Juli
dc.date.accessioned2022-03-29T14:00:08Z
dc.date.available2022-03-29T14:00:08Z
dc.date.issued2015
dc.description.abstractPremise of the study: Endemic plants on oceanic islands have long served as model systems for studying patterns and processes of evolution. However, phylogenetic studies of island plants frequently illustrate a decoupling of molecular divergence and ecological/morphological diversity, resulting in phylogenies lacking the resolution required to interpret patterns of evolution in a phylogenetic context. The current study uses the primarily Macaronesian fl owering plant genus Tolpis to illustrate the utility of multiplexed shotgun genotyping (MSG) for resolving relationships at relatively deep (among archipelagos) and very shallow (within archipelagos) nodes in this small, yet diverse insular plant lineage that had not been resolved with other molecular markers. • Methods: Genomic libraries for 27 accessions of Macaronesian Tolpis were generated for genotyping individuals using MSG, a form of reduced-representation sequencing, similar to restriction-site-associated DNA markers (RADseq). The resulting data fi les were processed using the program pyRAD, which clusters MSG loci within and between samples. Phylogenetic analyses of the aligned data matrix were conducted using RAxML. • Key results: Analysis of MSG data recovered a highly resolved phylogeny with generally strong support, including the fi rst robust inference of relationships within the highly diverse Canary Island clade of Tolpis . • Conclusions: The current study illustrates the utility of MSG data for resolving relationships in lineages that have undergone recent, rapid diversifi cation resulting in extensive ecological and morphological diversity. We suggest that a similar approach may prove generally useful for other rapid plant radiations where resolving phylogeny has been diffi cult.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMort, M. E., Crawford, D. J., Kelly, J. K., Santos‐Guerra, A., Sequeira, M. M., Moura, M., & Caujapé‐Castells, J. (2015). Multiplexed‐shotgun‐genotyping data resolve phylogeny within a very recently derived insular lineage. American Journal of Botany, 102(4), 634-641. https://doi.org/10.3732/ajb.1400551pt_PT
dc.identifier.doi10.3732/ajb.1400551pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4197
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBotanical Society of Americapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAsteraceaept_PT
dc.subjectCanary Islands (Spain)pt_PT
dc.subjectGenome sequencingpt_PT
dc.subjectMacaronesiapt_PT
dc.subjectMultiplexed shotgun genotypingpt_PT
dc.subjectPhylogenomicspt_PT
dc.subjectPhylogenypt_PT
dc.subjectRadiationpt_PT
dc.subjectTolpispt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleMultiplexed-shotgun-genotyping data resolve phylogeny within a very recently derived insular lineagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage641pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage634pt_PT
oaire.citation.titleAmerican Journal of Botanypt_PT
oaire.citation.volume102pt_PT
person.familyNameSequeira
person.familyNameMoura
person.givenNameMiguel
person.givenNameMónica
person.identifier.ciencia-idCA1C-1AC6-6346
person.identifier.ciencia-idB61A-C1E6-2360
person.identifier.orcid0000-0001-9728-465X
person.identifier.orcid0000-0001-5555-2388
person.identifier.scopus-author-id35101791200
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc4c52673-d265-42c0-b5bd-628a3b2f0b0b
relation.isAuthorOfPublicationb3f00f07-59c6-4308-be22-73b4d0dfdef1
relation.isAuthorOfPublication.latestForDiscoveryc4c52673-d265-42c0-b5bd-628a3b2f0b0b

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