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Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam

2021Journal article Open access
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dc.contributor.authorAndrade, Carlos G.
dc.contributor.authorSilva, Emanuel M. da
dc.contributor.authorRagonezi, Carla
dc.contributor.authorCarvalho, Miguel Â. A. Pinheiro de
dc.date.accessioned2022-02-04T10:35:02Z
dc.date.available2022-02-04T10:35:02Z
dc.date.issued2021
dc.description.abstractIpomoea batatas (L.) Lam. commonly known as sweet potato, is an important staple food worldwide, mainly due to its high nutritional value and yield. However, vegetative reproduction of sweet potato makes it more susceptible to viral infections, which threatens its productivity, quality, and difficult long-term preservation in germplasm banks. Also, it can act as a virus reservoir infecting the rest of the plant accessions in the bank collections. Hence, this work aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars from the germplasm collection of the ISOPlexis Germplasm Bank, Madeira, Portugal. The infection prevalence by these viruses among cultivars was 81.25%, 25.00%, and 6.25%, respectively; being ISOP1011 the only accession coinfected by Potyvirus and Carlavirus. The accessions ISOP1006, ISOP1010, and ISOP1047 were also coinfected by Begomovirus and Potyvirus, highlighting their vulnerability to viral infections. The ISOP1005 and ISOP1027 accessions were the only ones not infected by any of these viruses. The analysis of the partial sequence obtained from the Carlavirus detected in the accession ISOP1011, revealed the existence of an ORF that encodes for 93 amino acids of the catalytic domain of an RNA-directed RNA polymerase related to the Tymovirus protein family, as could be confirmed by comparison with proteins stored in UniProtKB. Multiple sequence alignment with these proteins showed that Motifs A and B of the catalytic domain were conserved. The search for sequence similarity with sequences deposited in GenBank reported a high sequence identity with Sweet potato yellow mottle virus (SPYMV) and Sweet potato chlorotic fleck virus (SPCFV). However, the 9-11% discrepancy in nucleotide sequence identity and a phylogenetic analysis carried out using the maximum probability method suggests the virus isolated from ISOP1011 is a new divergent strain of the SPCFV species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAndrade, C. G., Da Silva, E. M., Ragonezi, C., & Carvalho, M. Â. P. (2021). Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12222-12222. http://dx.doi.org/10.15835/nbha49112222.pt_PT
dc.identifier.doi10.15835/nbha49112222pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4040
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademicPrespt_PT
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCarlaviruspt_PT
dc.subjectCultivarspt_PT
dc.subjectSweet potatopt_PT
dc.subjectGermplasm bankpt_PT
dc.subjectMolecular diagnosispt_PT
dc.subjectRdRppt_PT
dc.subjectPhylogenypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.subjectEscola Superior de Tecnologias e Gestão
dc.titleViral diagnosis in cultivars of Ipomoea batatas (L.) Lampt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage12222pt_PT
oaire.citation.titleNotulae Botanicae Horti Agrobotanici Cluj-Napocapt_PT
oaire.citation.volume49pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Silva
person.familyNameRagonezi
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameEmanuel Marques
person.givenNameCarla
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-idFC12-D3F0-EF3D
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0002-2505-1045
person.identifier.orcid0000-0002-1822-5473
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id36460033800
person.identifier.scopus-author-id8758577600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverya51cc988-63ce-40e5-9b71-a27b1eadc937
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