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Quantification of δ-, γ- and α-Tocopherol in tomatoes using an improved liquid-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatography

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dc.contributor.authorFigueira, José A.
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-24T14:25:26Z
dc.date.available2019-07-24T14:25:26Z
dc.date.issued2017
dc.description.abstractTomato (Solanum lycopersicum L.) consumption has been correlated with a lower incidence of cardiovascular diseases and cancer. This protective effect has been ascribed to different bioactive compounds present in this fruit. Therefore, to gain insights on the potential of S. lycopersicum L. as bioactive food, a fast and sensitive methodology, based on liquid–liquid extraction (LLE), dispersive solid phase extraction (dSPE) followed by ultrahigh pressure liquid chromatography (UHPLC-FLR) analysis, was developed and validated to quantify δ-, γ- and α-tocopherol in tomatoes. Upon the optimization of different parameters, a fast extraction and separation, and simultaneously, increased resolution and sensitivity was attained. The methodology was validated, retrieving better analytical performance than most methods reported so far. This included good linearity, (r 2 > 0.99) and precision (<6.4%), high recoveries (>79.5%) and improved limits of detection and quantification (LODs of 2.15, 5.52 and 1.67 ng/mL and LOQs of 7.18, 18.40 and 5.58 ng/mL, for δ- γ- and α-tocopherol, respectively). These limits are about 1000 times lower than those reported in literature. Furthermore, as far we are aware, this is the first time δ-tocopherol presence in tomato is fully characterized and quantified. The methodology was applied to different tomato varieties, ripening stages and fruit sections, revealing high levels of δ-tocopherol that increase along fruit ripening, while the α-tocopherol follows the inverse trend. Moreover, δ-tocopherol is almost fully concentrated in the seeds and skin of ripe tomato. Finally, ORAC and DPPH assays revealed that the selected tocopherols contribute to approximately half of tomato total antioxidant capacity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFigueira, J. A., Pereira, J. A., & Câmara, J. S. (2017). Quantification of δ-, γ- and α-Tocopherol in tomatoes using an improved liquid-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatography. Food Analytical Methods, 10(7), 2507-2517.pt_PT
dc.identifier.doi10.1007/s12161-017-0799-0pt_PT
dc.identifier.issn1936-9751
dc.identifier.urihttp://hdl.handle.net/10400.13/2507
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.subjectLLE-dSPEpt_PT
dc.subjectUHPLC-FLRpt_PT
dc.subjectδ- γ- and α--tocopherolspt_PT
dc.subjectMethod validationpt_PT
dc.subjectSolanum lycopersicum L.pt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleQuantification of δ-, γ- and α-Tocopherol in tomatoes using an improved liquid-dispersive solid-phase extraction combined with ultrahigh pressure liquid chromatographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66177%2F2009/PT
oaire.citation.endPage2517pt_PT
oaire.citation.startPage2507pt_PT
oaire.citation.titleFood Analytical Methodspt_PT
oaire.citation.volume10(7)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameOliveira Figueira
person.familyNameAugusto Machado Pereira
person.familyNameCâmara
person.givenNameJosé Aldónio
person.givenNameJorge
person.givenNameJosé
person.identifierA-8724-2017
person.identifierG-3003-2013
person.identifier.ciencia-id0B1E-B3DD-95F5
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-3719-9327
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57201813505
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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