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Screening of salivary volatiles for putative breast cancer discrimination: an exploratory study involving geographically distant populations

2018-07Journal article Restricted access
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dc.contributor.authorCavaco, Carina
dc.contributor.authorPereira, Jorge A. M.
dc.contributor.authorTaunk, Khushman
dc.contributor.authorTaware, Ravindra
dc.contributor.authorRapole, Srikanth
dc.contributor.authorNagarajaram, Hampapathalu
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-25T10:53:55Z
dc.date.available2019-07-25T10:53:55Z
dc.date.issued2018-07
dc.description.abstractSaliva is possibly the easiest biofluid to analyse and, despite its simple composition, contains relevant metabolic information. In this work, we explored the potential of the volatile composition of saliva samples as biosignatures for breast cancer (BC) non-invasive diagnosis. To achieve this, 106 saliva samples of BC patients and controls in two distinct geographic regions in Portugal and India were extracted and analysed using optimised headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME/GC-MS, 2 mL acidified saliva containing 10% NaCl, stirred (800 rpm) for 45 min at 38 °C and using the CAR/PDMS SPME fibre) followed by multivariate statistical analysis (MVSA). Over 120 volatiles from distinct chemical classes, with significant variations among the groups, were identified. MVSA retrieved a limited number of volatiles, viz. 3-methyl-pentanoic acid, 4-methyl-pentanoic acid, phenol and p-tert-butyl-phenol (Portuguese samples) and acetic, propanoic, benzoic acids, 1,2-decanediol, 2-decanone, and decanal (Indian samples), statistically relevant for the discrimination of BC patients in the populations analysed. This work defines an experimental layout, HS-SPME/GC-MS followed by MVSA, suitable to characterise volatile fingerprints for saliva as putative biosignatures for BC non-invasive diagnosis. Here, it was applied to BC samples from geographically distant populations and good disease separation was obtained. Further studies using larger cohorts are therefore very pertinent to challenge and strengthen this proof-of-concept study.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCavaco, C., Pereira, J. A., Taunk, K., Taware, R., Rapole, S., Nagarajaram, H., & Câmara, J. S. (2018). Screening of salivary volatiles for putative breast cancer discrimination: an exploratory study involving geographically distant populations. Analytical and bioanalytical chemistry, 410(18), 4459-4468.pt_PT
dc.identifier.doi10.1007/s00216-018-1103-xpt_PT
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10400.13/2510
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.relationEXPLORING THE VOLATOME OF NONCOMMUNICABLE DISEASES AS A PROMISING, INNOVATIVE AND INTEGRATING APPROACH FOR THEIR RAPID DIAGNOSTICS. THE CASE STUDY OF CANCER AND NEURODEGENERATIVE DISEASES
dc.subjectSalivapt_PT
dc.subjectVolatile organic metabolitespt_PT
dc.subjectHS-SPMEpt_PT
dc.subjectGC-MSpt_PT
dc.subjectBreast cancer diagnosispt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleScreening of salivary volatiles for putative breast cancer discrimination: an exploratory study involving geographically distant populationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardTitleEXPLORING THE VOLATOME OF NONCOMMUNICABLE DISEASES AS A PROMISING, INNOVATIVE AND INTEGRATING APPROACH FOR THEIR RAPID DIAGNOSTICS. THE CASE STUDY OF CANCER AND NEURODEGENERATIVE DISEASES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/New-INDIGO%2F0003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/Inn-INDIGO%2F0001%2F2015/PT
oaire.citation.endPage4468pt_PT
oaire.citation.startPage4459pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume410(18)pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameAugusto Machado Pereira
person.familyNameTAUNK
person.familyNameTaware
person.familyNameCâmara
person.givenNameJorge
person.givenNameKHUSHMAN
person.givenNameRavindra
person.givenNameJosé
person.identifierG-3003-2013
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0003-2293-2936
person.identifier.orcid0000-0001-7893-2850
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57195429801
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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