Logo do repositório
 
Publicação

Towards scalable production of bound extracellular polymeric substances (B-EPS): autoclave hydrothermal extraction coupled with solvent-free ultrafiltration

2026Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosEngenharia e Tecnologia::Outras Engenharias e Tecnologias
dc.contributor.authorMendonça, Ivana
dc.contributor.authorRodrigues, Filipa
dc.contributor.authorMarisa Faria
dc.contributor.authorGómez Pinchetti, Juan L.
dc.contributor.authorFerreira, Artur
dc.contributor.authorCordeiro, Nereida
dc.contributor.authorSilva Mendonça, Ivana Rita da
dc.contributor.authorGonçalves Rodrigues, Ana Filipa
dc.contributor.authorFaria, Marisa Camacho Gonçalves
dc.contributor.authorcordeiro, nereida
dc.date.accessioned2026-09-21T14:36:39Z
dc.date.available2026-09-21T14:36:39Z
dc.date.issued2026
dc.description.abstractBound extracellular polymeric substances (B-EPS) are extracellular polysaccharides tightly attached to cyanobacterial and microalgal cell surfaces, representing a high-value class of biopolymers with industrial potential. Selective extraction is technically challenging due to strong adhesion to the cell wall and poten tial co-extraction of soluble EPS. Conventional methods can be chemically aggressive and may involve high energy and/or solvent inputs, making solvent-free extraction routes desirable. In this work, four hydrothermal extraction techniques (reflux, autoclave, ultrasonic bath, and microwave) were evaluated for their ability to recover B-EPS from the marine cyanobacterium Chroococcus submarinus (BEA 1200B), followed by a harmonised ultrafiltration step. Each method was assessed for extraction efficiency and its impact on bulk descriptors (inorganic carry-over, ATR-FTIR, zeta potential, and thermal profiles) and mor phology. Among the methods tested, autoclave extraction demonstrated the highest performance, yield ing up to 2.5 times more B-EPS than the other methods and showing reduced inorganic carry-over after purification. Across all methods, the purified B-EPS fractions exhibited broadly comparable bulk profiles under the applied analytics. Response Surface Methodology (RSM) applied to the autoclave system ident ified temperature and extraction time as key variables; optimal conditions (biomass-to-solvent ratio 1:20 (w/v), 130 °C, 16 min) enabled >90% recovery. Coupling autoclave extraction with solvent-free ultrafiltra tion avoids solvent precipitation and the use of hazardous reagents, enabling desalting and removal of low-molecular-weight components. Using a photosynthetic marine strain supports seawater cultivation and biogenic CO2 uptake, aligning the workflow with carbon-mitigation goals.eng
dc.identifier.citationIvana Mendonça, Filipa Rodrigues, Marisa Faria, Juan L. Gómez Pinchetti, Artur Ferreira, Nereida Cordeiro; Towards scalable production of bound extracellular polymeric substances (B-EPS): autoclave hydrothermal extraction coupled with solvent-free ultrafiltration. Green Chem. 2026; 28 (9): 4152–4163. https://doi.org/10.1039/d6gc00485g
dc.identifier.doi10.1039/d6gc00485g
dc.identifier.issn1463-9262
dc.identifier.issn1463-9270
dc.identifier.urihttp://hdl.handle.net/10400.13/7959
dc.language.isoeng
dc.peerreviewedyes
dc.publisherRoyal Society of Chemistry (RSC)
dc.relationDeveloping Bioactive and Intelligent Biomaterials from Microalgae/Cyanobacteria for Sustainable Food Packaging
dc.relationUID/PRR/04423/2025
dc.relationLA/P/0101/2020
dc.relation2023.04389.BD
dc.relation2024.01262.BD
dc.relation2020.06615.BD
dc.relation.ispartofGreen Chemistry
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectExtracellular polymeric substances (B-EPS)
dc.subjectAutoclave hydrothermal extraction
dc.subject.
dc.subjectFaculdade de Ciências Exatas e da Engenharia
dc.titleTowards scalable production of bound extracellular polymeric substances (B-EPS): autoclave hydrothermal extraction coupled with solvent-free ultrafiltrationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumber2023.04389.BD
oaire.awardTitleDeveloping Bioactive and Intelligent Biomaterials from Microalgae/Cyanobacteria for Sustainable Food Packaging
oaire.awardURIhttp://hdl.handle.net/10400.13/7509
oaire.citation.endPage4163
oaire.citation.issue9
oaire.citation.startPage4152
oaire.citation.titleGreen Chemistry
oaire.citation.volume28
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameSilva Mendonça
person.familyNameGonçalves Rodrigues
person.familyNameFaria
person.familyNamecordeiro
person.givenNameIvana Rita da
person.givenNameAna Filipa
person.givenNameMarisa Camacho Gonçalves
person.givenNamenereida
person.identifier3435316
person.identifier1612636
person.identifier.ciencia-id7619-C648-AE6C
person.identifier.ciencia-idA816-656A-6465
person.identifier.ciencia-idD716-6F48-AD0E
person.identifier.ciencia-id521A-0F62-EA39
person.identifier.orcid0000-0002-9148-026X
person.identifier.orcid0000-0001-7171-6012
person.identifier.orcid0000-0002-9158-6961
person.identifier.orcid0000-0001-6006-3415
person.identifier.ridO-2253-2019
person.identifier.scopus-author-id7004319456
relation.isAuthorOfPublication832d1d80-719b-4935-b565-1d7c31c90f77
relation.isAuthorOfPublication4b8b827d-260f-401e-9062-b09ddcdb82fa
relation.isAuthorOfPublication57ef72f0-8e40-4853-b4ab-416884e127fa
relation.isAuthorOfPublication3d02b657-c48c-4f56-813a-1284b81960f3
relation.isAuthorOfPublication.latestForDiscovery832d1d80-719b-4935-b565-1d7c31c90f77
relation.isProjectOfPublication4563bd17-f203-46bf-931e-b2641f10e7ab
relation.isProjectOfPublication.latestForDiscovery4563bd17-f203-46bf-931e-b2641f10e7ab

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Towards scalable production of bound extracellular polymeric substances.pdf
Tamanho:
2.16 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

Artigos em revistas internacionais