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Orientador(es)
Resumo(s)
Bound extracellular polymeric substances (B-EPS) are extracellular polysaccharides tightly attached to
cyanobacterial and microalgal cell surfaces, representing a high-value class of biopolymers with industrial
potential. Selective extraction is technically challenging due to strong adhesion to the cell wall and poten
tial co-extraction of soluble EPS. Conventional methods can be chemically aggressive and may involve
high energy and/or solvent inputs, making solvent-free extraction routes desirable. In this work, four
hydrothermal extraction techniques (reflux, autoclave, ultrasonic bath, and microwave) were evaluated for
their ability to recover B-EPS from the marine cyanobacterium Chroococcus submarinus (BEA 1200B),
followed by a harmonised ultrafiltration step. Each method was assessed for extraction efficiency and its
impact on bulk descriptors (inorganic carry-over, ATR-FTIR, zeta potential, and thermal profiles) and mor
phology. Among the methods tested, autoclave extraction demonstrated the highest performance, yield
ing up to 2.5 times more B-EPS than the other methods and showing reduced inorganic carry-over after
purification. Across all methods, the purified B-EPS fractions exhibited broadly comparable bulk profiles
under the applied analytics. Response Surface Methodology (RSM) applied to the autoclave system ident
ified temperature and extraction time as key variables; optimal conditions (biomass-to-solvent ratio 1:20
(w/v), 130 °C, 16 min) enabled >90% recovery. Coupling autoclave extraction with solvent-free ultrafiltra
tion avoids solvent precipitation and the use of hazardous reagents, enabling desalting and removal of
low-molecular-weight components. Using a photosynthetic marine strain supports seawater cultivation
and biogenic CO2 uptake, aligning the workflow with carbon-mitigation goals.
Descrição
Palavras-chave
Extracellular polymeric substances (B-EPS) Autoclave hydrothermal extraction . Faculdade de Ciências Exatas e da Engenharia
Contexto Educativo
Citação
Ivana Mendonça, Filipa Rodrigues, Marisa Faria, Juan L. Gómez Pinchetti, Artur Ferreira, Nereida Cordeiro; Towards scalable production of bound extracellular polymeric substances (B-EPS): autoclave hydrothermal extraction coupled with solvent-free ultrafiltration. Green Chem. 2026; 28 (9): 4152–4163. https://doi.org/10.1039/d6gc00485g
Editora
Royal Society of Chemistry (RSC)