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Publication
Effects of hydroxycinnamic acids on the glycolysis pathway
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Advisor(s)
Abstract(s)
Glycolysis is a metabolic pathway vital to the production of energy and some organisms rely on it solely to meet
their energy requirements. It is also a central pathway in the metabolism of carbohydrates and a source of
therapeutic targets against diabetes and cancer. Caffeoylquinic acids (CQAs) have been extensively studied for
their role in the treatment and prevention of diabetes (and cancer) but their mechanisms of action remain mostly
unknown. As such, molecular docking was used to find possible targets of CQAs in the glycolysis pathway. The
molecular docking assays showed that CQAs were docked preferably to the Rossman fold (nicotinamide adenine
dinucleotide — NAD(H) binding site) of oxidoreductases, that use NAD(H) as a cofactor, than to any other site.
In-vitro assays were then performed using two NAD(H) dependent oxidoreductases from glycolysis (alcohol
dehydrogenase and L-lactate dehydrogenase) in order confirm if CQAs would compete with the cofactor to
inhibit the reaction. The results from these assays indicate that CQAs can act as both inhibitors and activators
of NAD(H) dependent oxidoreductases of the glycolysis pathway.
Description
Keywords
Hydroxycinnamic acids Molecular docking Enzyme inhibition In vitro kinetic assays Glycolysis . Faculdade de Ciências Exatas e da Engenharia
Citation
Serina, J., Fernandes, M. X., & Castilho, P. C. (2019). Effects of hydroxycinnamic acids on the glycolysis pathway. South African Journal of Botany, 120, 219-229. 10.1016/j.sajb.2018.06.016
Publisher
South African Association of Botanists