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Effects of hydroxycinnamic acids on the glycolysis pathway

2019Journal article Open access
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dc.contributor.authorSerina, J.
dc.contributor.authorFernandes, M. X.
dc.contributor.authorCastilho, P. C.
dc.date.accessioned2021-03-25T14:54:57Z
dc.date.available2021-03-25T14:54:57Z
dc.date.issued2019
dc.description.abstractGlycolysis is a metabolic pathway vital to the production of energy and some organisms rely on it solely to meet their energy requirements. It is also a central pathway in the metabolism of carbohydrates and a source of therapeutic targets against diabetes and cancer. Caffeoylquinic acids (CQAs) have been extensively studied for their role in the treatment and prevention of diabetes (and cancer) but their mechanisms of action remain mostly unknown. As such, molecular docking was used to find possible targets of CQAs in the glycolysis pathway. The molecular docking assays showed that CQAs were docked preferably to the Rossman fold (nicotinamide adenine dinucleotide — NAD(H) binding site) of oxidoreductases, that use NAD(H) as a cofactor, than to any other site. In-vitro assays were then performed using two NAD(H) dependent oxidoreductases from glycolysis (alcohol dehydrogenase and L-lactate dehydrogenase) in order confirm if CQAs would compete with the cofactor to inhibit the reaction. The results from these assays indicate that CQAs can act as both inhibitors and activators of NAD(H) dependent oxidoreductases of the glycolysis pathway.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSerina, J., Fernandes, M. X., & Castilho, P. C. (2019). Effects of hydroxycinnamic acids on the glycolysis pathway. South African Journal of Botany, 120, 219-229. 10.1016/j.sajb.2018.06.016pt_PT
dc.identifier.doi10.1016/j.sajb.2018.06.016pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3203
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSouth African Association of Botanistspt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectHydroxycinnamic acidspt_PT
dc.subjectMolecular dockingpt_PT
dc.subjectEnzyme inhibitionpt_PT
dc.subjectIn vitro kinetic assayspt_PT
dc.subjectGlycolysispt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.titleEffects of hydroxycinnamic acids on the glycolysis pathwaypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.citation.endPage229pt_PT
oaire.citation.startPage219pt_PT
oaire.citation.titleSouth African Journal of Botanypt_PT
oaire.citation.volume120pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFernandes
person.familyNameCastilho
person.givenNameMiguel Xavier
person.givenNamePaula C
person.identifierA-4412-2013
person.identifier.ciencia-idED1D-3C7A-467C
person.identifier.ciencia-id6D15-8C69-A2E0
person.identifier.orcid0000-0002-1840-616X
person.identifier.orcid0000-0002-8303-4286
person.identifier.ridA-4373-2013
person.identifier.scopus-author-id35466972500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationd84fd917-438e-43bf-ac6f-256562481c05
relation.isAuthorOfPublication.latestForDiscovery8dab9a0d-f44a-4d2d-b9b1-7b3145162ca3
relation.isProjectOfPublication75aaadc4-cd17-4fec-ba9d-1d0e564b3731
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