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Microchip electrophoretic analysis of Phaseolin patterns and Its comparison with currently used SDS-PAGE techniques
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Orientador(es)
Resumo(s)
The goal of this work was to compare reproducibility
of phaseolin patterns of common bean obtained
by two electrophoretic protein separation techniques
including the conventional SDS-PAGE and an automated
chip electrophoresis system. Five standard cultivars of
common bean provided by the United States Department
of Agriculture (Beltsville, Maryland) that represented
five phaseolin types, T (Tendergreen), C (Contender) and
S (Sanilac), B (Boyaca) and P (Pampa), were used in this
study. Comparison of the phaseolin patterns revealed that
the chip-on-a-lab electrophoresis provided a good reproducibility.
The phaseolin polymorphism included four to
seven polypeptides typical for the pattern composition of
the T, C and S types. The polymorphism of the B and P
patterns was also established. Phaseolin polypeptides separated
by the microchip electrophoresis exhibited differences
with respect to the molecular weights and
electrophoretic mobility as compared to the SDS-PAGE
technique. This phenomenon could be attributed to the
absence of a solid separation phase in the microchip electrophoresis.
Moreover, this technique has potential to
substantially accelerate screening of large bean germplasm
collections since it allows for the accurate analysis of the
higher number of individual plants within accessions than
the conventional, tedious and time consuming SDS-PAGE
method.
Descrição
Palavras-chave
Microchip electrophoresis SDS-PAGE Technique validation Protein pattern Phaseolin . Faculdade de Ciências da Vida
Contexto Educativo
Citação
Silva, E. M., Santos, T. M. M., Ganança, J. F. T., Slaski, J. J., & Carvalho, M. Â. P. (2013). Microchip electrophoretic analysis of phaseolin patterns and its comparison with currently used SDS-PAGE techniques. Chromatographia, 76(17-18), 1163-1169. DOI 10.1007/s10337-013-2511-x
Editora
Springer