Repository logo
 
Publication

Microchip electrophoretic analysis of Phaseolin patterns and Its comparison with currently used SDS-PAGE techniques

2013Journal article Open access
Simple item page
dc.contributor.authorSilva, Emanuel Marques da
dc.contributor.authorSantos, Teresa Maria Marques dos
dc.contributor.authorGanança, José Filipe Teixeira
dc.contributor.authorSlaski, Jan Jacek
dc.contributor.authorCarvalho, Miguel Â. A. Pinheiro de
dc.date.accessioned2021-03-12T15:47:46Z
dc.date.available2021-03-12T15:47:46Z
dc.date.issued2013
dc.description.abstractThe goal of this work was to compare reproducibility of phaseolin patterns of common bean obtained by two electrophoretic protein separation techniques including the conventional SDS-PAGE and an automated chip electrophoresis system. Five standard cultivars of common bean provided by the United States Department of Agriculture (Beltsville, Maryland) that represented five phaseolin types, T (Tendergreen), C (Contender) and S (Sanilac), B (Boyaca) and P (Pampa), were used in this study. Comparison of the phaseolin patterns revealed that the chip-on-a-lab electrophoresis provided a good reproducibility. The phaseolin polymorphism included four to seven polypeptides typical for the pattern composition of the T, C and S types. The polymorphism of the B and P patterns was also established. Phaseolin polypeptides separated by the microchip electrophoresis exhibited differences with respect to the molecular weights and electrophoretic mobility as compared to the SDS-PAGE technique. This phenomenon could be attributed to the absence of a solid separation phase in the microchip electrophoresis. Moreover, this technique has potential to substantially accelerate screening of large bean germplasm collections since it allows for the accurate analysis of the higher number of individual plants within accessions than the conventional, tedious and time consuming SDS-PAGE method.pt_PT
dc.description.sponsorshipAt the USDA-United States Department of Agriculture for ceding species Phaseolus vulgaris L. standards used in this studypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, E. M., Santos, T. M. M., Ganança, J. F. T., Slaski, J. J., & Carvalho, M. Â. P. (2013). Microchip electrophoretic analysis of phaseolin patterns and its comparison with currently used SDS-PAGE techniques. Chromatographia, 76(17-18), 1163-1169. DOI 10.1007/s10337-013-2511-xpt_PT
dc.identifier.doi10.1007/s10337-013-2511-xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3167
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicrochip electrophoresispt_PT
dc.subjectSDS-PAGEpt_PT
dc.subjectTechnique validationpt_PT
dc.subjectProtein patternpt_PT
dc.subjectPhaseolinpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleMicrochip electrophoretic analysis of Phaseolin patterns and Its comparison with currently used SDS-PAGE techniquespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1169pt_PT
oaire.citation.issue17-18pt_PT
oaire.citation.startPage1163pt_PT
oaire.citation.titleChromatographiapt_PT
oaire.citation.volume76pt_PT
person.familyNameda Silva
person.familyNameGanança
person.familyNameAlmeida Pinheiro de Carvalho
person.givenNameEmanuel Marques
person.givenNameJosé Filipe
person.givenNameMiguel Angelo
person.identifier1218558
person.identifier.ciencia-id4610-6741-6816
person.identifier.orcid0000-0002-2505-1045
person.identifier.orcid0000-0002-8384-6188
person.identifier.orcid0000-0002-5084-870X
person.identifier.ridAEE-0913-2022
person.identifier.scopus-author-id8758577600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication21d8a5a0-c756-4057-994e-6aba453ce764
relation.isAuthorOfPublicationd5a8bb82-d9e9-4b7d-8852-e65a5931b17d
relation.isAuthorOfPublication2e433c48-88f8-40a2-b21f-de78b44aab1c
relation.isAuthorOfPublication.latestForDiscoveryd5a8bb82-d9e9-4b7d-8852-e65a5931b17d

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Microchip electrophoretic analysis of phaseolin patterns and its comparison with currently .pdf
Size:
306.11 KB
Format:
Adobe Portable Document Format
Download

Collections

Artigos em revistas internacionais