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Advisor(s)
Abstract(s)
Chitinases (EC 3.2.1.14) catalyse the hydrolysis of chitin, a homopolymer of
b-1,4-linked N-acetyl-D-glucosamine residues. Plant chitinases are involved in
a wide variety of processes; in particular, their expression has been found to be
enhanced in symbiotic and pathogenic plant–microbe interactions. During this
work we have cloned and characterized a gene encoding a class III chitinase from
actinorhizal nodules of Casuarina glauca (cgchi3). CGCHI3 was found to be
encoded by a single gene that was specifically activated in nodules as compared
with uninoculated control roots and leaves. The expression of this gene was
further enhanced in nodules after salicylic acid treatment and completely
repressed after wounding. In situ hybridisation analysis revealed that cgchi3 is
an early nodulin gene, being expressed in the meristem and in the uninfected
cortical cells of young nodules. Based on the obtained results we suggest that this
gene is involved in nodule development. This is the first report on a class III
chitinase coding gene that is specifically activated during actinorhizal symbiosis.
Description
Keywords
cgchi3 Casuarina glauca Chitinase . Faculdade de Ciências da Vida
Citation
Fortunato, A., Santos, P., Graça, I., Gouveia, M. M., Martins, S. M., Pinto Ricardo, C. P., ... & Ribeiro, A. (2007). Isolation and characterization of cgchi3, a nodule‐specific gene from Casuarina glauca encoding a class III chitinase. Physiologia Plantarum, 130(3), 418-426.
Publisher
Wiley