Green extraction approach based on μSPEed® followed by HPLC-MS/MS  for the determination of atropine and scopolamine in tea and herbal  tea infusions

González-Gómez, Lorena; Pereira, Jorge A. M.; Morante-Zarcero, Sonia; Câmara, José S.; Sierra, Isabel; Augusto Machado Pereira, Jorge; Câmara, José

http://hdl.handle.net/10400.13/7302

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Autores

González-Gómez, Lorena

Pereira, Jorge A. M.

Morante-Zarcero, Sonia

Câmara, José S.

Sierra, Isabel

Augusto Machado Pereira, Jorge

Câmara, José

Resumo(s)

liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been proposed for the analysis of atropine and scopolamine in tea and herbal tea infusions. For this, a digiVOL® Digital Syringe was used with different sorbents and working conditions to obtain a fast and efficient µSPEed® extraction. The best performance was achieved with a PS/DVB sorbent phase, sample loading of 5 × 500 µL and elution with 2 × 100 µL aliquots of methanol. The strategy based on µSPEed® followed by HPLC-MS/MS was validated, attaining quantitation limits lower than 0.15 ng mL− 1 and recoveries between 94 and 106% for both analytes and applied to seventeen tea and herbal tea infusions. Fourteen infusions showed contamination with one or both analytes above the maximum content legislated (sum of atropine and scopolamine < 0.2 ng mL− 1).

Palavras-chave

μSPEed® Atropine Scopolamine Tea and herbal teas Infusions HPLC-MS/MS . Centro de Química da Madeira Faculdade de Ciências Exatas e da Engenharia

URI

http://hdl.handle.net/10400.13/7302

Citação

González-Gómez, L., Pereira, J. A., Morante-Zarcero, S., Câmara, J. S., & Sierra, I. (2022). Green extraction approach based on μSPEed® followed by HPLC-MS/MS for the determination of atropine and scopolamine in tea and herbal tea infusions. Food Chemistry, 394, 133512.