Publication
Green extraction approach based on μSPEed® followed by HPLC-MS/MS for the determination of atropine and scopolamine in tea and herbal tea infusions
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.75 MB | Adobe PDF |
Advisor(s)
Abstract(s)
liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been proposed for the analysis of atropine
and scopolamine in tea and herbal tea infusions. For this, a digiVOL® Digital Syringe was used with different
sorbents and working conditions to obtain a fast and efficient µSPEed® extraction. The best performance was
achieved with a PS/DVB sorbent phase, sample loading of 5 × 500 µL and elution with 2 × 100 µL aliquots of
methanol. The strategy based on µSPEed® followed by HPLC-MS/MS was validated, attaining quantitation limits
lower than 0.15 ng mL− 1 and recoveries between 94 and 106% for both analytes and applied to seventeen tea
and herbal tea infusions. Fourteen infusions showed contamination with one or both analytes above the
maximum content legislated (sum of atropine and scopolamine < 0.2 ng mL− 1).
Description
Keywords
μSPEed® Atropine Scopolamine Tea and herbal teas Infusions HPLC-MS/MS . Centro de Química da Madeira Faculdade de Ciências Exatas e da Engenharia
Citation
González-Gómez, L., Pereira, J. A., Morante-Zarcero, S., Câmara, J. S., & Sierra, I. (2022). Green extraction approach based on μSPEed® followed by HPLC-MS/MS for the determination of atropine and scopolamine in tea and herbal tea infusions. Food Chemistry, 394, 133512.
Publisher
Elsevier