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Effect of chemical stress imposed by Solanum nigrum in calreticulin and beta-1,4- endoglucanase genes and in infectivity of Pratylenchus goodeyi

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dc.contributor.authorPestana, M.
dc.contributor.authorAbrantes, I.
dc.contributor.authorGouveia, M.
dc.date.accessioned2023-07-26T13:49:48Z
dc.date.available2023-07-26T13:49:48Z
dc.date.issued2014
dc.description.abstractIsolation and expression of effector genes encoding proteins secreted by plant-parasitic nematodes into a host can be helpful in improving the understand ing of parasitic interactions. In this study, calreticulin, a highly conserved Ca2+-binding and multifunctional pro tein, and beta-1,4-endoglucanase, a cell wall-degrading enzyme, both known to be secreted from oesophageal gland cells and injected through the nematode stylet into host tissue, were analysed. Full-length cDNAs from calreticulin (crt) and beta-1,4-endoglucanase (eng) with an estimated size of 1549 and 1342 bp, respectively, were isolated from the root-lesion nematode Pratylenchus goodeyi (Pg) by RT-PCR and RACE tech niques. Pg-crt and Pg-eng cDNAs were characterized in silico, and their expression assessed by semi quantitative PCR in nematodes exposed to a chemical stress provided by a Solanum nigrum extract showing nematicidal activity. It was demonstrated that the plant extract down-regulated the levels of Pg-crt mRNA, whereas the transcripts of Pg-eng mRNA held steady. This extract also affected nematode behaviour towards the roots since the number of nematodes that reached and penetrated the roots diminished when the exposure time rose. These observations indicate that the nemati cidal compounds present in the plant extract were effec tive as a signal to influence the infection success of P. goodeyi in vitro and it might be tested against other phytoparasitic nematodes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPestana, M., Abrantes, I., & Gouveia, M. (2015). Effect of chemical stress imposed by Solanum nigrum in calreticulin and beta-1, 4-endoglucanase genes and in infectivity of Pratylenchus goodeyi. European Journal of Plant Pathology, 141, 747-759.pt_PT
dc.identifier.doi10.1007/s10658-014-0575-6pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5292
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectBanana plantpt_PT
dc.subjectBeta-1,4-endoglucanasept_PT
dc.subjectCalreticulinpt_PT
dc.subjectChemical stresspt_PT
dc.subjectGene expressionpt_PT
dc.subjectRootlesion nematodept_PT
dc.subjectSolanum nigrumpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleEffect of chemical stress imposed by Solanum nigrum in calreticulin and beta-1,4- endoglucanase genes and in infectivity of Pratylenchus goodeyipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage759pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage747pt_PT
oaire.citation.titleEuropean Journal of Plant Pathologypt_PT
oaire.citation.volume141pt_PT
person.familyNameGouveia
person.givenNameManuela
person.identifier.ciencia-idA017-CFB6-50D5
person.identifier.orcid0000-0001-5758-2230
person.identifier.scopus-author-id16480248200
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione7211b14-d59d-49b9-8a5c-b44291e0ea77
relation.isAuthorOfPublication.latestForDiscoverye7211b14-d59d-49b9-8a5c-b44291e0ea77

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