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pH-sensitive Laponite®/doxorubicin/alginate nanohybrids with improved anticancer efficacy

2014Journal article Restricted access
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dc.contributor.authorGonçalves, Mara
dc.contributor.authorFigueira, Priscilla
dc.contributor.authorMaciel, Dina
dc.contributor.authorRodrigues, João
dc.contributor.authorQu, Xue
dc.contributor.authorLiu, Changsheng
dc.contributor.authorTomás, Helena
dc.contributor.authorLi, Yulin
dc.date.accessioned2019-06-21T14:30:29Z
dc.date.available2019-06-21T14:30:29Z
dc.date.issued2014
dc.description.abstractThe efficacy of the anticancer drug doxorubicin (Dox) is limited by an insufficient cellular uptake and drug resistance, which is partially due to ion trapping in acidic environments such as the extracellular environment of solid tumors and the interior of endolysosome vesicles. Herein, we describe the preparation and in vitro evaluation of a new type of nanohybrid for anticancer drug delivery which is capable of carrying a high load of the cationic Dox through the cell membrane. In addition, the nanohybrids use the acidic environment of the endolysosomes to release the drug, simultaneously helping to disrupt the endolysosomes and diminishing endolysosome Dox trapping. Furthermore, as the nanohybrid carriers are capable of sustained drug delivery, those that remain in the cytoplasm and still contain Dox are expected to exert a prolonged anticancer activity. Briefly, Dox is loaded onto biocompatible anionic Laponite(®) (LP) nanodisks with a high aspect ratio (25 nm in diameter and 0.92 nm in thickness) through strong electrostatic interactions to get Dox-loaded LP disks. Alginate (AG), a biocompatible natural polymer, is then coated onto the Dox-loaded LP disks (LP/Dox/AG nanohybrids) to prevent the burst release of the drug. The results demonstrate that the nanohybrids have a high encapsulation efficiency (80.8 ± 10.6%), are sensitive to pH and display a sustained drug release behavior. Cell culture experiments indicate that the LP/Dox/AG nanohybrids can be effectively internalized by CAL-72 cells (an osteosarcoma cell line), and exhibit a remarkable higher cytotoxicity to cancer cells than the free Dox. The merits of Laponite(®)/alginate nanohybrids, such as biocompatibility, high loading capacity and stimulus responsive release of cationic chemotherapeutic drugs, render them as excellent platforms for drug delivery.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGonçalves, M., Figueira, P., Maciel, D., Rodrigues, J., Qu, X., Liu, C., ... & Li, Y. (2014). pH-sensitive Laponite®/doxorubicin/alginate nanohybrids with improved anticancer efficacy. Acta biomaterialia, 10(1), 300-307.pt_PT
dc.identifier.doi10.1016/j.actbio.2013.09.013pt_PT
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/10400.13/2419
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
dc.relationSELF-ASSEMBLED NANOPARTICLES BASED ON DENDRIMER-POLYETHYLENE GLYCOL-POLYESTER BUILDING BLOCKS FOR DUAL GENE AND DRUG DELIVERY
dc.subjectAlginatept_PT
dc.subjectLaponitept_PT
dc.subjectDoxorubicinpt_PT
dc.subjectPH-sensitive nanohybridspt_PT
dc.subjectDrug deliverypt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titlepH-sensitive Laponite®/doxorubicin/alginate nanohybrids with improved anticancer efficacypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSelf-assembled nanoparticles based on PEG-PLA-dendrimer building blocks for dual gene/drug delivery
oaire.awardTitleSELF-ASSEMBLED NANOPARTICLES BASED ON DENDRIMER-POLYETHYLENE GLYCOL-POLYESTER BUILDING BLOCKS FOR DUAL GENE AND DRUG DELIVERY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F116788%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCTM-NAN%2F112428%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F88721%2F2012/PT
oaire.citation.endPage307pt_PT
oaire.citation.startPage300pt_PT
oaire.citation.titleActa Biomaterialiapt_PT
oaire.citation.volume10(1)pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
person.familyNameGonçalves
person.familyNamePorto-Figueira
person.familyNameMaciel
person.familyNameRodrigues
person.familyNameTomás
person.familyNameLi
person.givenNameMara
person.givenNamePriscilla
person.givenNameDina
person.givenNameJoão
person.givenNameHelena
person.givenNameYulin
person.identifier556975
person.identifier.ciencia-id6010-1863-4B11
person.identifier.ciencia-idAD11-4860-FB1B
person.identifier.ciencia-id211C-6048-FB1A
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-0332-3548
person.identifier.orcid0000-0001-7773-182X
person.identifier.orcid0000-0001-8684-6100
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-7856-2041
person.identifier.orcid0000-0001-5569-1038
person.identifier.ridB-6816-2008
person.identifier.ridE-5991-2010
person.identifier.ridA-4082-2013
person.identifier.scopus-author-id55509603000
person.identifier.scopus-author-id56724173300
person.identifier.scopus-author-id54781586200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id6508104177
person.identifier.scopus-author-id55718977200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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