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Bioinspired hybrid DNA/dendrimer-based films with supramolecular chirality

2025Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Químicas
datacite.subject.fosEngenharia e Tecnologia::Engenharia dos Materiais
dc.contributor.authorCastro, Rita
dc.contributor.authorGranja, Pedro L.
dc.contributor.authorRodrigues, João
dc.contributor.authorPêgo, Ana Paula
dc.contributor.authorTomás, Helena
dc.contributor.authorCastro, Rita
dc.contributor.authorRodrigues, João
dc.contributor.authorPêgo, Ana Paula
dc.contributor.authorTomás, Helena
dc.date.accessioned2026-08-07T14:58:09Z
dc.date.available2026-08-07T14:58:09Z
dc.date.issued2025
dc.description.abstractBioinspired hybrid DNA/dendrimer films were obtained by heating long double-stranded DNA (dsDNA) above its melting temperature and, while in the denatured state, mixing it with poly(amidoamine) (PAMAM) dendrimers, followed by controlled cooling. The formation of these new types of films was found to be dependent on several parameters, including the initial heating temperature, pH, buffer composition, dendrimer generation, amine/phosphate (N/P) ratio, and cooling speed. In addition to the PAMAM dendrimers (generations 3, 4, and 5), films could also be produced with branched poly(ethylenimine) with a molecular weight of 25 kDa. The results indicated that these films were formed not only through electrostatic interactions established between the negatively charged DNA molecules and the positive dendrimers, as expected, but also through random rehybridization of the single-stranded DNA (ssDNA) during the cooling process. The resulting films are water-insoluble, transparent when thin, highly elastic when air-dried, exceptionally stable over extended periods, cytocompatible, and easily scalable. Notably, the slow cooling process allowed for the establishment of at least a partially ordered structure in the films, as revealed by circular dichroism, providing evidence of supramolecular chirality. It is envisioned that these films may have significant potential in biomedical applications, such as drug/gene delivery systems, platforms for cell-free DNA transcription and components in biosensors.eng
dc.identifier.citationCastro, R., Granja, P. L., Rodrigues, J., Pêgo, A. P., & Tomás, H. (2025). Bioinspired hybrid DNA/dendrimer-based films with supramolecular chirality. Journal of Materials Chemistry B, 13(15), 4671-4680.
dc.identifier.doi10.1039/d4tb02761b
dc.identifier.issn2050-750X
dc.identifier.issn2050-7518
dc.identifier.urihttp://hdl.handle.net/10400.13/7916
dc.language.isoeng
dc.peerreviewedyes
dc.publisherRoyal Society of Chemistry (RSC)
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.relationCONTROLLED ENZYMATIC ASSEMBLY OF SSDNA-FUNCTIONALIZED DENDRIMERS FOR BIOMEDICAL APPLICATIONS
dc.relation.ispartofJournal of Materials Chemistry B
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBioinspired hybrid DNA/dendrimer films
dc.subjectSupramolecular chirality
dc.subjectDendrimers
dc.subjectBioinspired hybrid DNA
dc.subject.
dc.subjectCentro de Química da Madeira
dc.subjectFaculdade de Ciências Exatas e da Engenharia
dc.titleBioinspired hybrid DNA/dendrimer-based films with supramolecular chiralityeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/00674/2020
oaire.awardNumberUIDP/00674/2020
oaire.awardNumberSFRH/BD/87465/2012
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleCONTROLLED ENZYMATIC ASSEMBLY OF SSDNA-FUNCTIONALIZED DENDRIMERS FOR BIOMEDICAL APPLICATIONS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F87465%2F2012/PT
oaire.citation.endPage4680
oaire.citation.issue15
oaire.citation.startPage4671
oaire.citation.titleJournal of Materials Chemistry B
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCastro
person.familyNameRodrigues
person.familyNamePêgo
person.familyNameTomás
person.givenNameRita
person.givenNameJoão
person.givenNameAna Paula
person.givenNameHelena
person.identifier164495
person.identifier556975
person.identifier.ciencia-id8314-881E-4986
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-id6114-92F0-E64C
person.identifier.ciencia-id4D14-D31E-A8BE
person.identifier.orcid0000-0002-9337-062X
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0001-5169-328X
person.identifier.orcid0000-0002-7856-2041
person.identifier.ridA-2497-2013
person.identifier.ridB-6816-2008
person.identifier.ridC-6942-2012
person.identifier.ridE-5991-2010
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id56053889300
person.identifier.scopus-author-id6508104177
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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