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Ultrasound-assisted liquid-liquid extraction followed by ultrahigh pressure liquid chromatography for the quantification of major carotenoids in tomato

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dc.contributor.authorFigueira, José A.
dc.contributor.authorPereira, Jorge A.M.
dc.contributor.authorPorto-Figueira, Priscilla
dc.contributor.authorCâmara, José S.
dc.date.accessioned2019-07-24T13:28:28Z
dc.date.available2019-07-24T13:28:28Z
dc.date.issued2017
dc.description.abstractLycopene and β-carotene, the main carotenoids present in different tomatoes varieties (gordal, cherry, roma and campari) of Solanum lycopersicum L. (Solanaceae), were investigated using ultrasound-assisted liquid-liquid extraction (LLUSAE) followed by ultra-high pressure liquid chromatography with PDA detection (UHPLC-PDA) analysis. Experimental parameters influencing the LLUSAE were optimized using an univariate design, resulting in a 30-min ACN/MeOH extraction by sonication of a lyophilized sample, followed by PSA/C18/MgSO4 clean-up and fast centrifugation before UPLC analysis. Using this greener methodology, high recoveries (above 97%), good linearity (r2 > 0.98) and improved sensitivity, with limits of detection and quantification of 24.0 and 80.0 ng/mL for lycopene and 3.0 and 9.9 ng/mL for β-carotene, respectively, were obtained. This sensitivity is about five times better than previously reported in literature, making LLUSAE/UHPLC-PDA a promising strategy for lycopene and β-carotene quantification in tomato and eventually in other matrices. The carotenoids studied, lycopene and β-carotene, were found at highest concentrations in the gordal tomato variety, followed by cherry, roma and campari (727.1, 342.2, 267.2 and 218.2 μg/g and 80.4, 44.0, 45.7 and 44.0 μg/g for lycopene and β-carotene, respectively). Additionally, an exponential increase of both carotenoids occurs during ripening and mainly in the skin and locular cavity of the gordal variety. These results provide further evidences of the potential of tomatoes as an interesting source of lycopene and β-carotene.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFigueira, J. A., Pereira, J. A., Porto-Figueira, P., & Câmara, J. S. (2017). Ultrasound-assisted liquid-liquid extraction followed by ultrahigh pressure liquid chromatography for the quantification of major carotenoids in tomato. Journal of Food Composition and Analysis, 57, 87-93.pt_PT
dc.identifier.doi10.1016/j.jfca.2016.12.022pt_PT
dc.identifier.issn0889-1575
dc.identifier.urihttp://hdl.handle.net/10400.13/2506
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 674 - 2014
dc.subjectTomatopt_PT
dc.subjectTomato maturationpt_PT
dc.subjectSolanum lycopersicum L.pt_PT
dc.subjectLycopenept_PT
dc.subjectβ-carotenept_PT
dc.subjectLL-USAEpt_PT
dc.subjectUHPLC-PDApt_PT
dc.subjectFood analysispt_PT
dc.subjectFood compositionpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleUltrasound-assisted liquid-liquid extraction followed by ultrahigh pressure liquid chromatography for the quantification of major carotenoids in tomatopt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 674 - 2014
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FQUI%2FUI0674%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66177%2F2009/PT
oaire.citation.endPage93pt_PT
oaire.citation.startPage87pt_PT
oaire.citation.titleJournal of Food Composition and Analysispt_PT
oaire.citation.volume57pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameOliveira Figueira
person.familyNameAugusto Machado Pereira
person.familyNamePorto-Figueira
person.familyNameCâmara
person.givenNameJosé Aldónio
person.givenNameJorge
person.givenNamePriscilla
person.givenNameJosé
person.identifierA-8724-2017
person.identifierG-3003-2013
person.identifier.ciencia-id0B1E-B3DD-95F5
person.identifier.ciencia-idEC12-EE8F-50E8
person.identifier.ciencia-idAD11-4860-FB1B
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.orcid0000-0003-3719-9327
person.identifier.orcid0000-0003-0316-5348
person.identifier.orcid0000-0001-7773-182X
person.identifier.orcid0000-0003-1965-3151
person.identifier.scopus-author-id57201813505
person.identifier.scopus-author-id56724173300
person.identifier.scopus-author-id10140393000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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