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Phenotyping the ideotypes of two maize landraces from Madeira archipelago using morpho-agronomic traits and zein pattern

2018Journal article Open access
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dc.contributor.authorCarvalho, Miguel Â. A. Pinheiro de
dc.contributor.authorSardinha, Carmen M. S.
dc.contributor.authorGouveia, Carla S.S.
dc.contributor.authorSilva, Emanuel M. da
dc.contributor.authorNóbrega, Humberto G. M.
dc.contributor.authorGanança, José F. T.
dc.contributor.authorNóbrega, Sónia R.
dc.contributor.authorSantos, Teresa M. M. dos
dc.date.accessioned2020-09-10T10:13:22Z
dc.date.available2020-09-10T10:13:22Z
dc.date.issued2018
dc.description.abstractMadeira Archipelago holds specific maize genetic resources whose populations, representing field diversity, were previously classified into four main landrace groups. The ISOPs 0061 and 0070, with common names of “Milho Sequeiro” and “Milho Branco” yellow and white maize, respectively, represent the ideotypes of two of these landraces. These ideotypes have now been analyzed using morpho-agronomic and biochemical traits, to phenotype these landraces. The variation of nine of the ten morpho-agronomic traits was used to separate the landraces ideotypes. However, the seven traits of seed biochemical composition did not allow to segregate the landraces ideotypes. This is not the case of zein pattern, which polymorphism shows to be specific for each ideotype. The original ideotypes populations were regenerated twice under low input conditions. Zein patterns of the original and regenerated accessions were compared, using two electrophoresis techniques. The lab-on-a-chip electrophoresis showed that the standard SDS-PAGE zein pattern with six fractions could be separated into eighteen (ISOP 0061) and twenty (ISOP 0070) protein bands. In contrast, no significant changes were detected in the zein pattern structure of the initial and regenerated accessions of both landraces ideotypes. The chip electrophoresis showed to be a suitable technique to screen and characterize a large number of individuals and accessions of the germplasm collections, due to its reproducibility. In particular, the zein patterns can be used to phenotype ideotypes and establish a formula representing their structure, allowing to detect changes in landrace structure, occurring as a result of gene bank management actions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalho, M. Â. P., Sardinha, C. M., Gouveia, C. S., da Silva, E. M., Nóbrega, H. G., Ganança, J. F., ... & dos Santos, T. M. (2018). Phenotyping the ideotypes of two maize landraces from Madeira archipelago using morpho-agronomic traits and zein pattern. Maydica, 63(3), 13.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/2881
dc.language.isoengpt_PT
dc.publisherUnità di Ricerca per la Valorizzazione qualitativa dei cerealipt_PT
dc.subjectChip electrophoresispt_PT
dc.subjectGenotypept_PT
dc.subjectLandracespt_PT
dc.subjectZea mays L.pt_PT
dc.subjectZein patternpt_PT
dc.subjectMadeira archipelago (Portugal)pt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titlePhenotyping the ideotypes of two maize landraces from Madeira archipelago using morpho-agronomic traits and zein patternpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMaydicapt_PT
oaire.citation.volume63(3)pt_PT
person.familyNameAlmeida Pinheiro de Carvalho
person.familyNameGouveia
person.familyNameda Silva
person.familyNameNóbrega
person.familyNameGanança
person.givenNameMiguel Angelo
person.givenNameCarla
person.givenNameEmanuel Marques
person.givenNameHumberto
person.givenNameJosé Filipe
person.identifier1218558
person.identifier.ciencia-id4610-6741-6816
person.identifier.ciencia-idAE1B-9773-68A8
person.identifier.orcid0000-0002-5084-870X
person.identifier.orcid0000-0001-9309-4613
person.identifier.orcid0000-0002-2505-1045
person.identifier.orcid0000-0001-8900-9340
person.identifier.orcid0000-0002-8384-6188
person.identifier.ridAEE-0913-2022
person.identifier.ridAAH-9928-2020
person.identifier.scopus-author-id8758577600
person.identifier.scopus-author-id55554091900
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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