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Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races
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Orientador(es)
Resumo(s)
Persea americana (avocado) represents one of the most demanded food products worldwide,
with an important impact in several agronomy-based economies. The avocado is one of the most salt sensitive and valuable crops. It is therefore necessary to use salt-tolerant varieties, such as the West
Indian, for cultivation in locations with soil salinity problems, such as the Canary Islands. Therefore,
characterization of avocado cultivars is in demand, as well as development of molecular tools able to
easily identify the main avocado cultivars and horticultural races. In the present work, inter-Primer
Binding Site (iPBS) and Inter-Retrotransposon Amplified Polymorphism (IRAP) techniques, which
are based on retrotransposon with Long Terminal Repeats (LTR), have been implemented for the
first time in P. americana, allowing the characterization of genetic variation among cultivars from the
three main horticultural races and the identification of potential P. americana LTR sequences. The
iPBS approach showed clear advantages over its technical implementation, and allowed a better
delimitation of horticultural races, especially when focused on West Indian cultivars. However, both
techniques generated reproducible genetic fingerprints that not only allowed genetic characterization
of each cultivar analyzed, but also revealed potential molecular markers for the identification of
avocado cultivars and horticultural races.
Descrição
Palavras-chave
P. americana Avocado LTR-retrotransposon iPBS IRAP Molecular markers . Centro em Agricultura Sustentável e Tecnologia Alimentar
Contexto Educativo
Citação
: Carracedo, M.G.; Alonso, S.B.; Cabrera, R.S.B.; Jiménez-Arias, D.; Pérez Pérez, J.A. Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races. Agronomy 2022, 12, 1510. https://doi.org/ 10.3390/agronomy12071510
Editora
MDPI