Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races

González Carracedo, Mario; Alonso, Samuel Bello; Cabrera, Rahil Salomé Brito; Jiménez-Arias, David; Pérez Pérez, José Antonio

http://hdl.handle.net/10400.13/5336

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Authors

González Carracedo, Mario

Alonso, Samuel Bello

Cabrera, Rahil Salomé Brito

Jiménez-Arias, David

Pérez Pérez, José Antonio

Abstract(s)

Persea americana (avocado) represents one of the most demanded food products worldwide, with an important impact in several agronomy-based economies. The avocado is one of the most salt sensitive and valuable crops. It is therefore necessary to use salt-tolerant varieties, such as the West Indian, for cultivation in locations with soil salinity problems, such as the Canary Islands. Therefore, characterization of avocado cultivars is in demand, as well as development of molecular tools able to easily identify the main avocado cultivars and horticultural races. In the present work, inter-Primer Binding Site (iPBS) and Inter-Retrotransposon Amplified Polymorphism (IRAP) techniques, which are based on retrotransposon with Long Terminal Repeats (LTR), have been implemented for the first time in P. americana, allowing the characterization of genetic variation among cultivars from the three main horticultural races and the identification of potential P. americana LTR sequences. The iPBS approach showed clear advantages over its technical implementation, and allowed a better delimitation of horticultural races, especially when focused on West Indian cultivars. However, both techniques generated reproducible genetic fingerprints that not only allowed genetic characterization of each cultivar analyzed, but also revealed potential molecular markers for the identification of avocado cultivars and horticultural races.

Keywords

P. americana Avocado LTR-retrotransposon iPBS IRAP Molecular markers . Centro em Agricultura Sustentável e Tecnologia Alimentar

URI

http://hdl.handle.net/10400.13/5336

Citation

: Carracedo, M.G.; Alonso, S.B.; Cabrera, R.S.B.; Jiménez-Arias, D.; Pérez Pérez, J.A. Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races. Agronomy 2022, 12, 1510. https://doi.org/ 10.3390/agronomy12071510