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Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races

2022Journal article Open access
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dc.contributor.authorGonzález Carracedo, Mario
dc.contributor.authorAlonso, Samuel Bello
dc.contributor.authorCabrera, Rahil Salomé Brito
dc.contributor.authorJiménez-Arias, David
dc.contributor.authorPérez Pérez, José Antonio
dc.date.accessioned2023-10-26T16:17:40Z
dc.date.available2023-10-26T16:17:40Z
dc.date.issued2022
dc.description.abstractPersea americana (avocado) represents one of the most demanded food products worldwide, with an important impact in several agronomy-based economies. The avocado is one of the most salt sensitive and valuable crops. It is therefore necessary to use salt-tolerant varieties, such as the West Indian, for cultivation in locations with soil salinity problems, such as the Canary Islands. Therefore, characterization of avocado cultivars is in demand, as well as development of molecular tools able to easily identify the main avocado cultivars and horticultural races. In the present work, inter-Primer Binding Site (iPBS) and Inter-Retrotransposon Amplified Polymorphism (IRAP) techniques, which are based on retrotransposon with Long Terminal Repeats (LTR), have been implemented for the first time in P. americana, allowing the characterization of genetic variation among cultivars from the three main horticultural races and the identification of potential P. americana LTR sequences. The iPBS approach showed clear advantages over its technical implementation, and allowed a better delimitation of horticultural races, especially when focused on West Indian cultivars. However, both techniques generated reproducible genetic fingerprints that not only allowed genetic characterization of each cultivar analyzed, but also revealed potential molecular markers for the identification of avocado cultivars and horticultural races.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citation: Carracedo, M.G.; Alonso, S.B.; Cabrera, R.S.B.; Jiménez-Arias, D.; Pérez Pérez, J.A. Development of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Races. Agronomy 2022, 12, 1510. https://doi.org/ 10.3390/agronomy12071510pt_PT
dc.identifier.doi10.3390/agronomy12071510pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5336
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectP. americanapt_PT
dc.subjectAvocadopt_PT
dc.subjectLTR-retrotransposonpt_PT
dc.subjectiPBSpt_PT
dc.subjectIRAPpt_PT
dc.subjectMolecular markerspt_PT
dc.subject.pt_PT
dc.subjectCentro em Agricultura Sustentável e Tecnologia Alimentarpt_PT
dc.titleDevelopment of Retrotransposon-Based Molecular Markers for Characterization of Persea americana (Avocado) Cultivars and Horticultural Racespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue7pt_PT
oaire.citation.startPage1510pt_PT
oaire.citation.titleAgronomypt_PT
oaire.citation.volume12pt_PT
person.familyNameGonzález
person.familyNameJiménez-Arias
person.givenNameMario
person.givenNameDavid
person.identifier1075244
person.identifier182222
person.identifier.orcid0000-0003-1386-0571
person.identifier.orcid0000-0003-2304-7373
person.identifier.scopus-author-id56911150700
person.identifier.scopus-author-id34880055200
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5cf4a60-cdd9-4cb4-94a2-9b6f62ed0e9f
relation.isAuthorOfPublicationce020bf7-9b0f-4338-9a7b-83035239c306
relation.isAuthorOfPublication.latestForDiscoveryce020bf7-9b0f-4338-9a7b-83035239c306

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