Browsing by Author "Soneji, Dharmesh"
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- Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosisPublication . Taware, Ravindra; Taunk, Khushman; Pereira, Jorge A. M.; Dhakne, Rahul; Kannan, Narayanan; Soneji, Dharmesh; Câmara, José S.; Nagarajaram, H. A.; Rapole, SrikanthIntroduction Head and neck cancer (HNC), like many other forms of cancer, is usually detected in advanced stages, causing poor survival outcomes. Lack of specific and sensi tive screening markers for early detection of HNC has wors ened the scenario for the patients as well as the clinicians. Therefore, identification of efficient, noninvasive and afford able screening marker/methodology with high specificity and sensitivity is imminent need of situation. Objectives This study aims to identify and characterize urinary volatomic alterations specific to HNC. Methods Volatomic analysis of urine samples collected from HNC patients (n=29) and healthy controls (n=31) was performed using headspace solid phase microextraction coupled to gas chromatography mass spectrometry (GC– MS). Both univariate and multivariate statistical approaches were used to investigate HNC specific volatomic alterations. Results Statistical analysis revealed a total of 28 metabo lites with highest contribution towards discrimination of HNC patients from healthy controls (VIP >1, p < 0.05, Log2 FC ≥0.58/≤−0.57). The discrimination efficiency and accuracy of urinary VOCs was ascertained by ROC curve analysis that allowed the identification of four metabolites viz. 2,6-dimethyl-7-octen-2-ol, 1-butanol, p-xylene and 4-methyl-2-heptanone with highest sensitivity and speci ficity to discriminate HNC patients from healthy controls. Further, the metabolic pathway analysis identified several dysregulated pathways in HNC patients and their detailed investigations could unravel novel mechanistic insights into the disease pathophysiology. Conclusion Overall, this study provides valuable finger print of the volatile profile of HNC patients, which in turn, might help in improving the current understanding of this form of cancer and lead to the development of non-invasive approaches for HNC diagnosis.
- A non-invasive approach to explore the discriminatory potential of the urinary volatilome of invasive ductal carcinoma of the breastPublication . Taunk, Khushman; Taware, Ravindra; More, Tushar H.; Porto-Figueira, Priscilla; Pereira, Jorge A. M.; Mohapatra, Rajkishore; Soneji, Dharmesh; Câmara, José S.; Nagarajaram, H. A.; Rapole, SrikanthWorldwide, breast invasive ductal carcinoma (IDC) accounts for the majority of the reported cases of this form of cancer. IDC effective management, as for any form of cancer, would greatly benefit from early diagnosis. This, however, due to various socio-economic reasons, is very far for the reality in developing countries like India, where cancer diagnosis is often carried out at late stages when disease management is troublesome. With the present work, we aim to evaluate a simple analytical methodology to identify a set of volatile organic compounds (VOCs) in urine samples, as a biosignature for IDC. Using solid-phase microextraction followed by gas chromatography/mass spectrometry, a panel of 14 urinary VOCs was found to discriminate IDC (n = 65) from a healthy control (HC) group (n = 70) through multivariate statistical treatments. Furthermore, metabolic pathway analysis revealed various dysregulated pathways involved in IDC patients hinting that their detailed investigations could lead to novel mechanistic insights into the disease pathophysiology. In addition, we validated the expression pattern of five of these VOCs namely 2-ethyl-1-hexanol, isolongifolenone, furan, dodecanoic acid, 2-methoxy-phenol in another external cohort of 59 urinary samples (IDC = 32 and HC = 27) and found their expression pattern to be consistent with the primary sample set. To our knowledge, this is the first study exploring breast IDC volatome alterations in Indian patients.
- Volatilomic insight of head and neck cancer via the effects observed on saliva metabolitesPublication . Taware, Ravindra; Taunk, Khushman; Pereira, Jorge A. M.; Shirolkar, Amey; Soneji, Dharmesh; Câmara, José S.; Nagarajaram, H. A.; Rapole, SrikanthHead and neck cancer (HNC) is a heterogeneous malignant disease with distinct global distribution. Metabolic adaptations of HNC are significantly gaining clinical interests nowadays. Here, we investigated effects of HNC on differential expression of volatile metabolites in human saliva. We applied headspace solid phase microextraction coupled with gas chromatography-mass spectrometry analysis of saliva samples collected from 59 human subjects (HNC - 32, Control - 27). We identified and quantified 48 volatile organic metabolites (VOMs) and observed profound effects of HNC on these metabolites. These effects were VOM specific and significantly differed in the biologically comparable healthy controls. HNC induced changes in salivary VOM composition were well attributed to in vivo metabolic effects. A panel of 15 VOMs with variable importance in projection (VIP) score >1, false discovery rate (FDR) corrected p-value < 0.05 and log2 fold change (log2 FC) value of ≥0.58/≤-0.58 were regarded as discriminatory metabolites of pathophysiological importance. Afterwards, receiver operator characteristic curve (ROC) projected certain VOMs viz., 1,4-dichlorobenzene, 1,2-decanediol, 2,5-bis1,1-dimethylethylphenol and E-3-decen-2-ol with profound metabolic effects of HNC and highest class segregation potential. Moreover, metabolic pathways analysis portrayed several dysregulated pathways in HNC, which enhanced our basic understanding on salivary VOM changes. Our observations could redefine several known/already investigated systemic phenomenons (e.g. biochemical pathways). These findings will inspire further research in this direction and may open unconventional avenues for non-invasive monitoring of HNC and its therapy in the future.