Loading...
Publication
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.72 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Introduction Head and neck cancer (HNC), like many
other forms of cancer, is usually detected in advanced stages,
causing poor survival outcomes. Lack of specific and sensi tive screening markers for early detection of HNC has wors ened the scenario for the patients as well as the clinicians.
Therefore, identification of efficient, noninvasive and afford able screening marker/methodology with high specificity
and sensitivity is imminent need of situation.
Objectives This study aims to identify and characterize
urinary volatomic alterations specific to HNC. Methods Volatomic analysis of urine samples collected
from HNC patients (n=29) and healthy controls (n=31)
was performed using headspace solid phase microextraction
coupled to gas chromatography mass spectrometry (GC–
MS). Both univariate and multivariate statistical approaches
were used to investigate HNC specific volatomic alterations.
Results Statistical analysis revealed a total of 28 metabo lites with highest contribution towards discrimination of
HNC patients from healthy controls (VIP >1, p < 0.05,
Log2 FC ≥0.58/≤−0.57). The discrimination efficiency and
accuracy of urinary VOCs was ascertained by ROC curve
analysis that allowed the identification of four metabolites
viz. 2,6-dimethyl-7-octen-2-ol, 1-butanol, p-xylene and
4-methyl-2-heptanone with highest sensitivity and speci ficity to discriminate HNC patients from healthy controls.
Further, the metabolic pathway analysis identified several
dysregulated pathways in HNC patients and their detailed
investigations could unravel novel mechanistic insights into
the disease pathophysiology.
Conclusion Overall, this study provides valuable finger print of the volatile profile of HNC patients, which in turn,
might help in improving the current understanding of this
form of cancer and lead to the development of non-invasive
approaches for HNC diagnosis.
Description
Keywords
Head and neck cancer Volatomics VOMs GC–MS Urinary markers . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Taware, R., Taunk, K., Pereira, J. A., Dhakne, R., Kannan, N., Soneji, D., ... & Rapole, S. (2017). Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis. Metabolomics, 13(10), 1-11. DOI 10.1007/s11306-017-1251-6
Publisher
Springer