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Polyphenols, antioxidant potential and color of fortified wines during accelerated ageing: the Madeira wine case study
Publication . Pereira, Vanda; Albuquerque, Francisco; Cacho, Juan; Marques, José C.
Polyphenols, antioxidant potential and color of three types of fortified Madeira
wines were evaluated during the accelerated ageing, named as estufagem. The traditional
estufagem process was set to 45 °C for 3 months. Overheating conditions, 1 month at 70 °C,
were also examined. Total polyphenols (TP), total monomeric anthocyanins (TMA) and
total flavonoids (TF) were assessed by spectrophotometric methods, while individual
polyphenols and furans were simultaneously determined by HPLC-DAD. Antioxidant
potential (AP) was estimated by ABTS, DPPH and FRAP assays, while color was
evaluated by Glories and CIELab. Traditional estufagem decreased the TP and AP up to
20% and 26%, respectively, with final values similar to other wines. TMA of the Madeira
wines from red grapes decreased during estufagem. Six hydroxybenzoic acids, three
hydroxycinnamic acids, one stilbene, three flavonols and three flavan-3-ols were found in
these wines. The prominent phenolics were hydroxycinnamates and hydroxybenzoates,
even after estufagem. Most polyphenols decreased, with the exception of caffeic, ferulic,
p-coumaric, gallic and syringic acids. Finally, both chromatic systems revealed that all
wines tended to similar chromatic characteristics after estufagem. The study suggests that
estufagem can be applied without high impact on polyphenols and antioxidant potential of
these fortified wines.
Assessment of the development of browning, antioxidant activity and volatile organic compounds in thermally processed sugar model wines
Publication . Pereira, Vanda; Santos, Magda; Cacho, Juan; Marques, José C.
The study evaluates the contribution of the fructose and glucose's degradation for the Madeira wine's
features. The browning index, antioxidant activity and volatile organic compounds developed by the
glucose and fructose model systems simulating thermally processed sweet Madeira wines were assessed.
Sixteen different fructose/glucose model systems were prepared in synthetic wine and stored at 50 C for
4 months. Then, three model wines were also submitted to 70 C for 1 month. The browning index and
the antioxidant activity ranged between 0.00 and 0.27 AU and 3.0e65.3 mg(GAE)/L, respectively. The
development of several volatile organic compounds was demonstrated (up to 47). The identified com pounds were mostly furans, with 5-hydroxymethylfurfural as the most abundant. For the first time, it
was shown that the origin of sotolon in sweet wine can be associated with the acid-catalyzed fructose
degradation mechanism. Other 2(5H)-furanones were also identified. It could be concluded that part of
the browning, antioxidant activity and aroma compounds developed in sweet fortified wines is associ ated with the thermal degradation of fructose in acid medium.
Evolution of 5-hydroxymethylfurfural (HMF) and furfural (F) in fortified wines submitted to overheating conditions
Publication . Pereira, V.; Albuquerque, F. M.; Ferreira, A. C.; Cacho, J.; Marques, J. C.
As furfural (F) and 5-hydroxymethylfurfural (HMF) are essentially formed from sugar dehydration, especially
in food submitted to heat, they can be found in beverages, as well as fortified sweet wines. In order to assess
the impact of temperature on Madeira winemaking, three traditional varieties of Madeira wines (Malvasia,
Sercial and Tinta Negra Mole) were studied to evaluate the F and HMF contents. The wines were produced by
two vinification processes, following traditional and modern methodologies, heated at standard conditions
(30 °C and 45 °C, for 4 months) and compared with the same wines submitted to overheating conditions
(55 °C, for 4 months). The RP-HPLC-DAD methodology used for the control of F and HMF during the process
showed no significant changes in the wines maintained at 30 °C (canteiro) and a noticeable but controlled
increase in the wines heated at 45 °C (estufagem) where values up to about 150 mg/L of HMF could be found in
sweet wines. The strong relation of this compound with the sugar content and baking temperature stood out
in the wines submitted to overheating conditions where values higher than 1 g/L could be found for sweeter
wines, with HMF level being in general higher than F.
The results clearly suggest that the amount of HMF in these fortified wines can be easily controlled when
submitted to adequate conditions of heating during estufagem and storage. Furthermore, different
temperatures for the baking of sweet and dry wines may be considered.
Volatile profile of Madeira wines submitted to traditional accelerated ageing
Publication . Pereira, Vanda; Cacho, Juan; Marques, José C.
The evolution of monovarietal fortified Madeira wines forced-aged by traditional thermal processing
(estufagem) were studied in terms of volatiles. SPE extracts were analysed by GC–MS before and after
heating at 45 C for 3 months (standard) and at 70 C for 1 month (overheating). One hundred and ninety
volatile compounds were identified, 53 of which were only encountered in baked wines. Most chemical
families increased after standard heating, especially furans and esters, up to 61 and 3-fold, respectively.
On the contrary, alcohols, acetates and fatty acids decreased after heating. Varietal aromas, such as
Malvasia’s monoterpenic alcohols were not detected after baking. The accelerated ageing favoured the
development of some volatiles previously reported as typical aromas of finest Madeira wines, particularly
phenylacetaldeyde, b-damascenone and 5-ethoxymethylfurfural. Additionally, ethyl butyrate, ethyl 2-
methylbutyrate, ethyl caproate, ethyl isovalerate, guaiacol, 5-hydroxymethylfurfural and c-decalactone
were also found as potential contributors to the global aroma of baked wines.
Amino acids and biogenic amines evolution during the estufagem of fortified wines
Publication . Pereira, Vanda; Pereira, Ana C.; Pérez Trujillo, Juan P.; Cacho, Juan; Marques, José C.
The current study was focused on the impact of accelerated ageing (heating step) on the amino acid and biogenic amine profiles
of fortified wines. In this sense, three Madeira wines from two commonly used grape varieties (one red and the other white) were
analysed during the heating, at standard (45∘
C, 3 months) and overheating (70∘
C, 1 month) conditions, following a precolumn
derivatization procedure using iodoacetic acid, o-phthaldialdehyde, and 2-mercaptoethanol, carried out in the injection loop prior
to RP-HPLC-FLD detection. Eighteen amino acids were identified, with arginine being the most abundant. An important decrease
of the amino acid levels was detected during the standard heating (up to 30%), enhanced up to 61% by the temperature increase.
Cysteine, histidine, and asparagine revealed the greatest decreases at 45∘
C. Conversely, some amino acids, such as asparagine,
slightly increased. Four biogenic amines were identified but always in trace amounts. Finally, it was observed that the accelerated
ageing did not favour the biogenic amine development. The results also indicate that the heating process promotes the amino acid
transformation into new ageing products.
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Funding agency
Fundação para a Ciência e a Tecnologia
Funding programme
SFRH
Funding Award Number
SFRH/BD/24177/2005