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Publication
Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
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Advisor(s)
Abstract(s)
Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer
micropropagation to overcome the cessation of adventitious root development. In vitro inoculation
promotes the re-growth of the root system induced previously by auxin treatments, facilitating
acclimation and diminishing the losses of plants because of a weak root system that is incapable of
water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine
cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain
cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular
methods allowed fungi identification in all the mycorrhization phases and in the acclimation of
derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures
of roots were microscopically analyzed and compare with mycorrhizal roots.
Description
Keywords
Ectomycorrhiza Mycorrhiza-like structures Stone pine Adventitious roots Hartig net . Escola Superior de Tecnologias e Gestão
Citation
Ragonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps1030034
Publisher
MDPI