Publication
Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots
dc.contributor.author | Ragonezi, Carla | |
dc.contributor.author | Zavattieri, Maria | |
dc.date.accessioned | 2022-02-04T15:33:09Z | |
dc.date.available | 2022-02-04T15:33:09Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Several studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | Ragonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps1030034 | pt_PT |
dc.identifier.doi | 10.3390/mps1030034 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.13/4046 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | MDPI | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Ectomycorrhiza | pt_PT |
dc.subject | Mycorrhiza-like structures | pt_PT |
dc.subject | Stone pine | pt_PT |
dc.subject | Adventitious roots | pt_PT |
dc.subject | Hartig net | pt_PT |
dc.subject | . | pt_PT |
dc.subject | Escola Superior de Tecnologias e Gestão | pt_PT |
dc.title | Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-CFL%2F71437%2F2006/PT | |
oaire.citation.issue | 3 | pt_PT |
oaire.citation.title | Methods and Protocols | pt_PT |
oaire.citation.volume | 1 | pt_PT |
oaire.fundingStream | 5876-PPCDTI | |
person.familyName | Ragonezi | |
person.familyName | Zavattieri | |
person.givenName | Carla | |
person.givenName | Maria Amely | |
person.identifier.ciencia-id | FC12-D3F0-EF3D | |
person.identifier.orcid | 0000-0002-1822-5473 | |
person.identifier.orcid | 0000-0001-9640-8227 | |
person.identifier.scopus-author-id | 36460033800 | |
person.identifier.scopus-author-id | 35486257300 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
relation.isAuthorOfPublication | a51cc988-63ce-40e5-9b71-a27b1eadc937 | |
relation.isAuthorOfPublication | 4a188050-e705-41e2-af9c-2a67aecad273 | |
relation.isAuthorOfPublication.latestForDiscovery | a51cc988-63ce-40e5-9b71-a27b1eadc937 | |
relation.isProjectOfPublication | 6d373255-42e5-4099-9487-7e2aa0f611e8 | |
relation.isProjectOfPublication.latestForDiscovery | 6d373255-42e5-4099-9487-7e2aa0f611e8 |
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