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Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots

2018Journal article Open access
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dc.contributor.authorRagonezi, Carla
dc.contributor.authorZavattieri, Maria
dc.date.accessioned2022-02-04T15:33:09Z
dc.date.available2022-02-04T15:33:09Z
dc.date.issued2018
dc.description.abstractSeveral studies have shown the potential of using Ectomycorrhizal (ECM) fungi in conifer micropropagation to overcome the cessation of adventitious root development. In vitro inoculation promotes the re-growth of the root system induced previously by auxin treatments, facilitating acclimation and diminishing the losses of plants because of a weak root system that is incapable of water and nutrient absorption. During a series of mycorrhization experiments, cryostat and ultrafine cuts were used to study the morpho-histological transformation of the symbiotic roots. To obtain cryostat cuts from pine roots a method frequently used for animal tissue was adopted. Molecular methods allowed fungi identification in all the mycorrhization phases and in the acclimation of derived plants. Mycorrhizal-like-structures derived from in vitro culture and axenic liquid cultures of roots were microscopically analyzed and compare with mycorrhizal roots.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRagonezi, C., & Zavattieri, M. A. (2018). Histological studies of mycorrhized roots and mycorrhizal-like-structures in pine roots. Methods and Protocols, 1(3), 34. https://doi.org/10.3390/mps1030034pt_PT
dc.identifier.doi10.3390/mps1030034pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4046
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEctomycorrhizapt_PT
dc.subjectMycorrhiza-like structurespt_PT
dc.subjectStone pinept_PT
dc.subjectAdventitious rootspt_PT
dc.subjectHartig netpt_PT
dc.subject.pt_PT
dc.subjectEscola Superior de Tecnologias e Gestãopt_PT
dc.titleHistological studies of mycorrhized roots and mycorrhizal-like-structures in pine rootspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-CFL%2F71437%2F2006/PT
oaire.citation.issue3pt_PT
oaire.citation.titleMethods and Protocolspt_PT
oaire.citation.volume1pt_PT
oaire.fundingStream5876-PPCDTI
person.familyNameRagonezi
person.familyNameZavattieri
person.givenNameCarla
person.givenNameMaria Amely
person.identifier.ciencia-idFC12-D3F0-EF3D
person.identifier.orcid0000-0002-1822-5473
person.identifier.orcid0000-0001-9640-8227
person.identifier.scopus-author-id36460033800
person.identifier.scopus-author-id35486257300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa51cc988-63ce-40e5-9b71-a27b1eadc937
relation.isAuthorOfPublication4a188050-e705-41e2-af9c-2a67aecad273
relation.isAuthorOfPublication.latestForDiscoverya51cc988-63ce-40e5-9b71-a27b1eadc937
relation.isProjectOfPublication6d373255-42e5-4099-9487-7e2aa0f611e8
relation.isProjectOfPublication.latestForDiscovery6d373255-42e5-4099-9487-7e2aa0f611e8

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