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Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: validation and options to enhance the sensitivity
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Advisor(s)
Abstract(s)
We studied the possibility of detection of [Ru(η5
-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model
system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic
salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and
intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest
that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the
best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach
demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI
detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calcu lations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was
established, and higher sensitivity explained.
Description
Keywords
RuCp Metallodrugs Carbon dots MALDI-TOF MS Urine DFT calculations . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Nunes, N., Popović, I., Abreu, E., Maciel, D., Rodrigues, J., Soto, J., ... & Petković, M. (2021). Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: validation and options to enhance the sensitivity. Talanta, 222, 121551. https://doi.org/10.1016/j.talanta.2020.121551
Publisher
Elsevier