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Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: validation and options to enhance the sensitivity

2021Journal article Open access
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dc.contributor.authorNunes, Nádia
dc.contributor.authorPopović, Iva
dc.contributor.authorAbreu, Elder
dc.contributor.authorMaciel, Dina
dc.contributor.authorRodrigues, João
dc.contributor.authorSoto, Juan
dc.contributor.authorAlgarra, Manuel
dc.contributor.authorPetkovic, Marijana
dc.date.accessioned2022-02-17T11:33:57Z
dc.date.available2022-02-17T11:33:57Z
dc.date.issued2021
dc.description.abstractWe studied the possibility of detection of [Ru(η5 -C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calcu lations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was established, and higher sensitivity explained.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Popović, I., Abreu, E., Maciel, D., Rodrigues, J., Soto, J., ... & Petković, M. (2021). Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: validation and options to enhance the sensitivity. Talanta, 222, 121551. https://doi.org/10.1016/j.talanta.2020.121551pt_PT
dc.identifier.doi10.1016/j.talanta.2020.121551pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/4089
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.relationMetallodendrimers as HIV antiviral and anticancer agents.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectRuCppt_PT
dc.subjectMetallodrugspt_PT
dc.subjectCarbon dotspt_PT
dc.subjectMALDI-TOF MSpt_PT
dc.subjectUrinept_PT
dc.subjectDFT calculationspt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleDetection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: validation and options to enhance the sensitivitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMetallodendrimers as HIV antiviral and anticancer agents.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F102123%2F2014/PT
oaire.citation.startPage121551pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume222pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameAbreu
person.familyNameMaciel
person.familyNameRodrigues
person.familyNamePetkovic
person.givenNameElder
person.givenNameDina
person.givenNameJoão
person.givenNameMarijana
person.identifierC-5257-2008
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person.identifier.ciencia-id211C-6048-FB1A
person.identifier.ciencia-idA81C-620E-DD6A
person.identifier.ciencia-idAD15-C921-5954
person.identifier.orcid0000-0002-7823-7118
person.identifier.orcid0000-0001-8684-6100
person.identifier.orcid0000-0003-4552-1953
person.identifier.orcid0000-0002-8354-995X
person.identifier.ridP-5093-2018
person.identifier.ridB-6816-2008
person.identifier.scopus-author-id54781586200
person.identifier.scopus-author-id9233278800
person.identifier.scopus-author-id7006986199
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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