Principal physicochemical methods used to characterize dendrimer molecule complexes used as genetic therapy agents, nanovaccines or drug carriers

Rolando Alberto, Rodriguez Fonseca; Rodrigues, João; Muñoz-Fernández, María de los Angeles; Martínez Muñoz, Alberto; Fragoso Vázquez, Manuel Jonathan; Correa-Basurto, José

http://hdl.handle.net/10400.13/2432

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Authors

Rolando Alberto, Rodriguez Fonseca

Rodrigues, João

Muñoz-Fernández, María de los Angeles

Martínez Muñoz, Alberto

Fragoso Vázquez, Manuel Jonathan

Correa-Basurto, José

Abstract(s)

Nanomedicine is the application of nanotechnology to medicine. This field is related to the study of nanodevices and nanomaterials applied to various medical uses, such as in improving the pharmacological properties of different molecules. Dendrimers are synthetic nanoparticles whose physicochemical properties vary according to their chemical structure. These molecules have been extensively investigated as drug nanocarriers to improve drug solubility and as sustained-release systems. New therapies such as gene therapy and the development of nanovaccines can be improved by the use of dendrimers. The biophysical and physicochemical characterization of nucleic acid/peptide-dendrimer complexes is crucial to identify their functional properties prior to biological evaluation. In that sense, it is necessary to first identify whether the peptide-dendrimer or nucleic aciddendrimer complexes can be formed and whether the complex can dissociate under the appropriate conditions at the target cells. In addition, biophysical and physicochemical characterization is required to determine how long the complexes remain stable, what proportion of peptide or nucleic acid is required to form the complex or saturate the dendrimer, and the size of the complex formed. In this review, we present the latest information on characterization systems for dendrimer-nucleic acid, dendrimer-peptide and dendrimer-drug complexes with several biotechnological and pharmacological applications.

Keywords

Nanovaccines Gene therapy Dendrimers Peptides Nucleic acids Functional properties characterization . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira

URI

http://hdl.handle.net/10400.13/2432

Citation

Rodríguez, F. R. A.; Rodrigues, J.; Muñoz-Fernández, M. A.; Martínez, M. A.; Fragoso, V. M. J.; Correa B. J. Principal Physicochemical Methods Used to Characterize Dendrimer Molecule Complexes Used as Genetic Therapy Agents, Nanovaccines or Drug Carriers. Curr. Pharm. Des. 2017, 23, 1-8.