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A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits

2021Journal article Open access
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dc.contributor.authorSilva, Catarina
dc.contributor.authorCâmara, José S.
dc.contributor.authorPerestrelo, Rosa
dc.date.accessioned2022-01-03T14:26:03Z
dc.date.available2022-01-03T14:26:03Z
dc.date.issued2021
dc.description.abstractTropical fruits are a rich source of phenolic compounds which are favorable in defending the human body against damage induced by free radicals (e.g., ROS, RNS). In the current work, a high throughput analytical approach based on a simple extraction procedure (QuEChERS-dSPE) combined with high-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MSn ) was used to establish the phenolic profile of tropical fruits. The proposed method showed good linearity (r2 ≥ 0.991), precision (RSD < 8 %), as well as low limits of detection (LOD ≤ 19.7 μg/L) and quantification (LOQ ≤ 65.8 μg/ L). Thirty-four phenolic compounds were identified as belonging to different chemical groups, from which only 6 were common to all tropical fruits. Pitanga showed the highest relative phenolic concentration (99.5 mg/100 g of fruit), with the passion fruit (17.5 mg/100 g of fruit) the lowest. Flavonols were the most predominant chemical group in tropical fruits, representing 77.9, 60.1, and 55.8% of the phenolic composition of pitanga, passion fruit and mango, respectively. The data obtained allow deep and comprehensive insights into the phenolic compo sition of tropical fruits in order to explore its potential bioactive activity. Nevertheless, in vivo assays using fruit extracts will be essential to recognize their potential health-promoting properties.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, C., Câmara, J. S., & Perestrelo, R. (2021). A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits. Journal of Food Composition and Analysis, 98, 103844. ttps://doi.org/10.1016/j.jfca.2021.103844pt_PT
dc.identifier.doi10.1016/j.jfca.2021.103844pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/3938
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationMadeira Chemistry Research Centre
dc.relationMadeira Chemistry Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPhenolic profilept_PT
dc.subjectTropical fruitspt_PT
dc.subjectQuEChERS-dSPEpt_PT
dc.subjectHPLC–DAD–ESI–MSnpt_PT
dc.subjectMethod validationpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências Exatas e da Engenhariapt_PT
dc.subjectCentro de Química da Madeira
dc.titleA high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruitspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardTitleMadeira Chemistry Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00674%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00674%2F2020/PT
oaire.citation.startPage103844pt_PT
oaire.citation.titleJournal of Food Composition and Analysispt_PT
oaire.citation.volume98pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSousa Luís
person.familyNameCâmara
person.familyNamePerestrelo
person.givenNameCatarina Grace
person.givenNameJosé
person.givenNameRosa
person.identifierC-1300-2019
person.identifierG-3003-2013
person.identifier1441319
person.identifier.ciencia-id9813-3B88-BA8D
person.identifier.ciencia-id481C-08CE-90E5
person.identifier.ciencia-id251A-D5F7-9E32
person.identifier.orcid0000-0002-3018-3165
person.identifier.orcid0000-0003-1965-3151
person.identifier.orcid0000-0002-7223-1022
person.identifier.ridI-2307-2014
person.identifier.scopus-author-id57194492726
person.identifier.scopus-author-id10140393000
person.identifier.scopus-author-id16686828800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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