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A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits
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Advisor(s)
Abstract(s)
Tropical fruits are a rich source of phenolic compounds which are favorable in defending the human body against
damage induced by free radicals (e.g., ROS, RNS). In the current work, a high throughput analytical approach
based on a simple extraction procedure (QuEChERS-dSPE) combined with high-performance liquid
chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MSn
) was used
to establish the phenolic profile of tropical fruits. The proposed method showed good linearity (r2 ≥ 0.991),
precision (RSD < 8 %), as well as low limits of detection (LOD ≤ 19.7 μg/L) and quantification (LOQ ≤ 65.8 μg/
L). Thirty-four phenolic compounds were identified as belonging to different chemical groups, from which only 6
were common to all tropical fruits. Pitanga showed the highest relative phenolic concentration (99.5 mg/100 g of
fruit), with the passion fruit (17.5 mg/100 g of fruit) the lowest. Flavonols were the most predominant chemical
group in tropical fruits, representing 77.9, 60.1, and 55.8% of the phenolic composition of pitanga, passion fruit
and mango, respectively. The data obtained allow deep and comprehensive insights into the phenolic compo sition of tropical fruits in order to explore its potential bioactive activity. Nevertheless, in vivo assays using fruit
extracts will be essential to recognize their potential health-promoting properties.
Description
Keywords
Phenolic profile Tropical fruits QuEChERS-dSPE HPLC–DAD–ESI–MSn Method validation . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Silva, C., Câmara, J. S., & Perestrelo, R. (2021). A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits. Journal of Food Composition and Analysis, 98, 103844. ttps://doi.org/10.1016/j.jfca.2021.103844
Publisher
Elsevier