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Publication
Genetic diversity in Hemileia vastatrixbased on RAPD markers
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Advisor(s)
Abstract(s)
Random amplified polymorphic DNA
(RAPD) was used to assess the genetic structure of
Hemileia vastatrix populations. Forty-five rust iso lates with different virulence spectra and from dif ferent hosts and geographical regions were ana lyzed. Out of 45 bands, generated with three RAPD
primers, 35 (78%) were polymorphic and scored as
molecular markers. Cluster analysis exhibits unstruc tured variability of this pathogen with regard to
physiological race, geographical origin or host. The
genotypic diversity (H9) inferred from Shannon’s in dex was higher than gene diversity (Ht), suggesting
that diversity is distributed among clonal lineages.
Estimates of gene diversity in Africa and Asia pop ulations were higher in total (Ht) as compared to
within population diversity (Hs). Genetic differen tiation was considerable among coffee rust isolates
from Africa (Gst 5 0.865) and Asia (Gst 5 0.768)
but not among isolates from South America (Gst 5
0.266). We concluded that genetic diversity in H.
vastatrix was moderately low and that the genetic
differentiation among populations shows that asex ual reproduction is likely to play an important role
in the population biology of this fungus. This should
be taken into account for the development of breed ing programs.
Description
Keywords
Coffee rust DNA polymorphism Genetic variability Molecular markers Population structure . Faculdade de Ciências da Vida
Citation
Gouveia, M. M. C., Ribeiro, A., Várzea, V. M., & Rodrigues Jr, C. J. (2005). Genetic diversity in Hemileia vastatrix based on RAPD markers. Mycologia, 97(2), 396-404.
Publisher
Taylor and Francis