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Molecular cloning and characterization of cDNA encoding a Translocon-Associated Protein (TRAPδ) from the root-lesion nematode Pratylenchus goodeyi

2014Journal article Restricted access
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dc.contributor.authorPestana, Margarida
dc.contributor.authorAbrantes, Isabel M. de O.
dc.contributor.authorGouveia, Manuela
dc.date.accessioned2023-07-26T13:07:54Z
dc.date.available2023-07-26T13:07:54Z
dc.date.issued2014
dc.description.abstractThe translocon-associated protein (TRAP) complex comprises four subunits (α, β, γ, δ) and is located in the endoplasmic reticulum membrane at translocation sites. The TRAP complex is required for the efficient translocation of substrates and to correct or eliminate misfolded proteins. In this study, we described the cloning and characterization of a cDNA encoding a TRAP from the phytoparasitic nematode Pratylenchus goodeyi (Pg). The full-length cDNA had an estimated size of 690 bp and encodes a 177 amino acid peptide. The deduced protein after sequence analysis codes for TRAPδ subunit homologous to TRAPδ from other nematodes. The Pg-TRAPδ had a signal peptide indi cating a possible involvement in the transport and binding of other proteins at the endoplasmic reticulum membrane. The increase in relative expression of Pg trapδ, assessed by semi-quantitative PCR, was induced over time in nematodes exposed to a nematostatic/ nematicide extract of Solanum nigrum, suggesting that this gene product might be influenced by response mechanisms to stress in P. goodeyi. This is the first report of the cloning and characterization of trap cDNA from plant endoparasitic nematodes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPestana, M., Abrantes, I. M. O., & Gouveia, M. (2014). Molecular cloning and characterization of cDNA encoding a Translocon-Associated Protein (TRAPδ) from the root-lesion nematode Pratylenchus goodeyi. European journal of plant pathology, 139, 289-298.pt_PT
dc.identifier.doi10.1007/s10658-013-0363-8pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.13/5291
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.subjectPhytoparasitic nematodept_PT
dc.subjectBanana plantpt_PT
dc.subjectGene expressionpt_PT
dc.subjectSignal peptidept_PT
dc.subjectTRAP complexpt_PT
dc.subject.pt_PT
dc.subjectFaculdade de Ciências da Vidapt_PT
dc.titleMolecular cloning and characterization of cDNA encoding a Translocon-Associated Protein (TRAPδ) from the root-lesion nematode Pratylenchus goodeyipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage292pt_PT
oaire.citation.startPage283pt_PT
oaire.citation.titleEuropean Journal of Plant Pathologypt_PT
oaire.citation.volume139pt_PT
person.familyNameGouveia
person.givenNameManuela
person.identifier.ciencia-idA017-CFB6-50D5
person.identifier.orcid0000-0001-5758-2230
person.identifier.scopus-author-id16480248200
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione7211b14-d59d-49b9-8a5c-b44291e0ea77
relation.isAuthorOfPublication.latestForDiscoverye7211b14-d59d-49b9-8a5c-b44291e0ea77

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