Loading...
Publication
Molecular cloning and characterization of cDNA encoding a Translocon-Associated Protein (TRAPĪ“) from the root-lesion nematode Pratylenchus goodeyi
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 940.45 KB | Adobe PDF |
Advisor(s)
Abstract(s)
The translocon-associated protein (TRAP)
complex comprises four subunits (Ī±, Ī², Ī³, Ī“) and is
located in the endoplasmic reticulum membrane at
translocation sites. The TRAP complex is required for
the efficient translocation of substrates and to correct or
eliminate misfolded proteins. In this study, we described
the cloning and characterization of a cDNA encoding a
TRAP from the phytoparasitic nematode Pratylenchus
goodeyi (Pg). The full-length cDNA had an estimated
size of 690 bp and encodes a 177 amino acid peptide.
The deduced protein after sequence analysis codes for
TRAPĪ“ subunit homologous to TRAPĪ“ from other
nematodes. The Pg-TRAPĪ“ had a signal peptide indi cating a possible involvement in the transport and
binding of other proteins at the endoplasmic reticulum
membrane. The increase in relative expression of Pg trapĪ“, assessed by semi-quantitative PCR, was induced over time in nematodes exposed to a nematostatic/
nematicide extract of Solanum nigrum, suggesting that
this gene product might be influenced by response
mechanisms to stress in P. goodeyi. This is the first
report of the cloning and characterization of trap
cDNA from plant endoparasitic nematodes.
Description
Keywords
Phytoparasitic nematode Banana plant Gene expression Signal peptide TRAP complex . Faculdade de CiĆŖncias da Vida
Citation
Pestana, M., Abrantes, I. M. O., & Gouveia, M. (2014). Molecular cloning and characterization of cDNA encoding a Translocon-Associated Protein (TRAPĪ“) from the root-lesion nematode Pratylenchus goodeyi. European journal of plant pathology, 139, 289-298.
Publisher
Springer