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Publication
Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
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Advisor(s)
Abstract(s)
A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix
was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The
results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary
system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both
COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by
MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl,
N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative
charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important
detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the
complex formation between CDs, Na+
, cholesterol and DHB, and it was supported by theoretical calcula tions. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical
excitation, dipole moment and oscillator strength positively affected the cholesterol detection by
MALDI in the presence of Na+.
Description
Keywords
Cholesterol MALDI TOF Carbon dots N-doped Carbon dots DFT calculations . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira
Citation
Houdová, D., Soto, J., Castro, R., Rodrigues, J., Pino-González, M. S., Petković, M., ... & Algarra, M. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. Journal of Colloid and Interface Science, 591, 373-383. https://doi.org/10.1016/j.jcis.2021.02.004
Publisher
Elsevier